LIG1 antibody (Internal Region)

Catalog No. ABIN6262965

The Rabbit Polyclonal anti-LIG1 antibody has been validated for WB, ELISA, IHC, IF and ICC. It is suitable to detect LIG1 in samples from Human, Rat and Mouse.

Quick Overview for LIG1 antibody (Internal Region) (ABIN6262965)

Target: LIG1 (Ligase I, DNA, ATP-Dependent (LIG1))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LIG1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-LIG1 Antibody

Binding Specificity: Internal Region

Specificity: LIG1 Antibody detects endogenous levels of total LIG1.

Predicted Reactivity: Pig,Zebrafish,Bovine,Horse,Sheep,Rabbit,Dog

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human LIG1, corresponding to a region within the internal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for LIG1

Target: LIG1 (Ligase I, DNA, ATP-Dependent (LIG1))

Alternative Name: LIG1

Background:

Description: DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair.

Gene: LIG1

Molecular Weight: 102kDa

Gene ID: 3978

UniProt: P18858

Pathways: Telomere Maintenance, DNA Damage Repair, DNA Replication, Synthesis of DNA