MAGEA2B antibody (C-Term)
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- Target See all MAGEA2B Antibodies
- MAGEA2B (Melanoma Antigen Family A, 2B (MAGEA2B))
- Binding Specificity
- C-Term
- Reactivity
- Human
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This MAGEA2B antibody is un-conjugated
- Application
- ELISA, Western Blotting (WB)
- Specificity
- MAGEA2 Antibody detects endogenous levels of total MAGEA2.
- Purification
- The antiserum was purified by peptide affinity chromatography using SulfoLinkTM Coupling Resin (Thermo Fisher Scientific).
- Immunogen
- A synthesized peptide derived from human MAGEA2, corresponding to a region within C-terminal amino acids.
- Isotype
- IgG
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- Application Notes
- WB 1:1000-3000, ELISA(peptide) 1:20000-1:40000
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- -20 °C
- Storage Comment
- Store at -20 °C. Stable for 12 months from date of receipt.
- Expiry Date
- 12 months
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- Target
- MAGEA2B (Melanoma Antigen Family A, 2B (MAGEA2B))
- Alternative Name
- MAGEA2, MAGEA2B (MAGEA2B Products)
- Synonyms
- MAGE2 antibody, MAGEA2 antibody, MAGE family member A2B antibody, MAGEA2B antibody
- Background
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Description: Reduces p53/TP53 transactivation function through recruitment of HDAC3 to p53/TP53 transcription sites. Also represses p73/TP73 activity. Proposed to enhance ubiquitin ligase activity of RING-type zinc finger-containing E3 ubiquitin-protein ligases. In vitro enhances ubiquitin ligase activity of TRIM28 and stimulates p53/TP53 ubiquitination by TRIM28 potentially in presence of Ubl-conjugating enzyme UBE2H. Proposed to act through recruitment and/or stabilization of the Ubl-conjugating enzyme (E2) at the E3:substrate complex. May play a role in embryonal development and tumor transformation or aspects of tumor progression. In vitro promotes cell viability in melanoma cell lines. Antigen recognized on a melanoma by autologous cytolytic T-lymphocytes. Negatively regulates acetylation and sumoylation of PML and represses PML-induced p53/TP53 acetylation and activation.
Gene: MAGEA2, MAGEA2B
- Molecular Weight
- 35 kDa
- Gene ID
- 266740, 4101
- UniProt
- P43356
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