CDC42BPB antibody (C-Term)

Catalog No. ABIN6263348

This Rabbit Polyclonal antibody specifically detects CDC42BPB in ELISA, WB, IHC, IF and ICC. It exhibits reactivity toward Human.

Quick Overview for CDC42BPB antibody (C-Term) (ABIN6263348)

Target: CDC42BPB (CDC42 Binding Protein Kinase beta (DMPK-Like) (CDC42BPB))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CDC42BPB antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-CDC42BPB Antibody

Binding Specificity: C-Term

Specificity: MRCKbeta Antibody detects endogenous levels of total MRCKbeta.

Predicted Reactivity: Pig,Zebrafish,Bovine,Horse,Sheep,Dog

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human MRCKbeta, corresponding to a region within C-terminal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:1000, IHC 1:200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for CDC42BPB

Target: CDC42BPB (CDC42 Binding Protein Kinase beta (DMPK-Like) (CDC42BPB))

Alternative Name: CDC42BPB

Background:

Description: Serine/threonine-protein kinase which is an important downstream effector of CDC42 and plays a role in the regulation of cytoskeleton reorganization and cell migration. Regulates actin cytoskeletal reorganization via phosphorylation of PPP1R12C and MYL9/MLC2 (PubMed:21457715, PubMed:21949762). In concert with MYO18A and LURAP1, is involved in modulating lamellar actomyosin retrograde flow that is crucial to cell protrusion and migration (PubMed:18854160). Phosphorylates PPP1R12A (PubMed:21457715). In concert with FAM89B/LRAP25 mediates the targeting of LIMK1 to the lamellipodium resulting in its activation and subsequent phosphorylation of CFL1 which is important for lamellipodial F-actin regulation (By similarity).

Gene: CDC42BPB

Molecular Weight: 194kDa

Gene ID: 9578

UniProt: Q9Y5S2