NF-kB p65 antibody

Catalog No. ABIN6263616

The Rabbit Polyclonal anti-NF-kB p65 antibody has been validated for WB, IHC, ELISA, IF and ICC. It is suitable to detect NF-kB p65 in samples from Human, Mouse, Rat and Zebrafish (Danio rerio). There are 14+ publications available.

Quick Overview for NF-kB p65 antibody (ABIN6263616)

Target: NF-kB p65 (NFkBP65) (Nuclear Factor-kB p65 (NFkBP65))

Reactivity: Human, Mouse, Rat, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NF-kB p65 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-NF-kB p65 Antibody

Specificity: NF-kappaB p65 Antibody detects endogenous levels of total NF-kappaB p65

Cross-Reactivity: Human, Mouse (Murine), Rat (Rattus), Zebrafish (Danio rerio)

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human NF-kappaB p65

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000 IHC 1:50-1:200 IF 1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline ,  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C.Stable for 12 months from date of receipt

Expiry Date: 12 months

References for anti-NF-kB p65 antibody (ABIN6263616)

Yu, Ye, Che, Wu, Qi, Song, Guo, Zhang, Wu, Ren, Li: "FGF21 exerts comparable pharmacological efficacy with Adalimumab in ameliorating collagen-induced rheumatoid arthritis by regulating systematic inflammatory response." in: Biomedicine & pharmacotherapy, Vol. 89, pp. 751-760, (2018) (PubMed).

Huang, Zhang, Hou, Wang, Liu, Zhang, Chen, Zhu: "LncRNA AK023391 promotes tumorigenesis and invasion of gastric cancer through activation of the PI3K/Akt signaling pathway." in: Journal of experimental & clinical cancer research : CR, Vol. 36, Issue 1, pp. 194, (2018) (PubMed).

Wang, Xu, Zhang, Li, Wang, Guo, Li, Wang, Li: "Improving hyperglycemic effect of FGF-21 is associated with alleviating inflammatory state in diabetes." in: International immunopharmacology, Vol. 56, pp. 301-309, (2018) (PubMed).

Ding, Shen, Li, Jiang, Shen: "Therapeutic mild hypothermia improves early outcomes in rats subjected to severe sepsis." in: Life sciences, Vol. 199, pp. 1-9, (2018) (PubMed).

Zheng, Feng, Jiang, Wu, Tang, Kuang, Zeng, Zhou, Liu: "Selenium deficiency impaired immune function of the immune organs in young grass carp (Ctenopharyngodon idella)." in: Fish & shellfish immunology, Vol. 77, pp. 53-70, (2018) (PubMed).

Wu, Zhu, Zhang, Yin, Kang, Cao, Tian, Lu, Liu: "EGFR‑associated pathways involved in traditional Chinese medicine (TCM)‑1‑induced cell growth inhibition, autophagy and apoptosis in prostate cancer." in: Molecular medicine reports, Vol. 17, Issue 6, pp. 7875-7885, (2018) (PubMed).

Zhu, Xu, Lv, He, Ye, Wang, Hu: "miR-199b-5p Regulates Immune-Mediated Allograft Rejection after Lung Transplantation Through the GSK3β and NF-κB Pathways." in: Inflammation, Vol. 41, Issue 4, pp. 1524-1535, (2018) (PubMed).

Li, Xiao, Yang, Qin, Gao, Liu, Zhou: "Parthenolide attenuated bleomycin-induced pulmonary fibrosis via the NF-κB/Snail signaling pathway." in: Respiratory research, Vol. 19, Issue 1, pp. 111, (2018) (PubMed).

Wu, Gu, Mei, Xu, Jing, Yao, Chen, Yang, Chen, Yang, Qi, Hu, Wüthrich, Mei: "Resveratrol delays polycystic kidney disease progression through attenuation of nuclear factor κB-induced inflammation." in: Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association, Vol. 31, Issue 11, pp. 1826-1834, (2017) (PubMed).

Wu, Wang, Wang, Yu, Wang, Song, Liu, Ye, Xu, Cao, Li, Ren: "The bispecific antibody aimed at the vicious circle of IL-1β and IL-17A, is beneficial for the collagen-induced rheumatoid arthritis of mice through NF-κB signaling pathway." in: Immunology letters, Vol. 179, pp. 68-79, (2017) (PubMed).

Ai, Liu, Lu, Liang, Sun, Chen, Sun, Li, Liu, Zhang, Liu, Xiao, Jing, Sun, Zhou, Yang: "Phenytoin silver: a new nanocompound for promoting dermal wound healing via comprehensive pharmacological action." in: Theranostics, Vol. 7, Issue 2, pp. 425-435, (2017) (PubMed).

Yu, Bai, Wang, Liu, Yuan, Qu, Zhang, Tian, Li, Li, Ren: "Fibroblast growth factor 21 protects mouse brain against D-galactose induced aging via suppression of oxidative stress response and advanced glycation end products formation." in: Pharmacology, biochemistry, and behavior, Vol. 133, pp. 122-31, (2016) (PubMed).

Su, Wang, Li, Yin, Hao, Lv, Li, Zhao, Du, Li, Zhang: "Overexpression of RBM5 induces autophagy in human lung adenocarcinoma cells." in: World journal of surgical oncology, Vol. 14, pp. 57, (2016) (PubMed).

Yu, Li, Liu, Tian, Yuan, Bai, Wang, Zhang, Ren, Zhang, Li: "Fibroblast growth factor 21 (FGF21) ameliorates collagen-induced arthritis through modulating oxidative stress and suppressing nuclear factor-kappa B pathway." in: International immunopharmacology, Vol. 25, Issue 1, pp. 74-82, (2015) (PubMed).

Target Details for NF-kB p65

Target: NF-kB p65 (NFkBP65) (Nuclear Factor-kB p65 (NFkBP65))

Alternative Name: NF-kappaB p65

Background:

Description: NF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52 and the heterodimeric p65-p50 complex appears to be most abundant one. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. NF-kappa-B heterodimeric p65-p50 and p65-c-Rel complexes are transcriptional activators. The NF-kappa-B p65-p65 complex appears to be involved in invasin-mediated activation of IL-8 expression. The inhibitory effect of I-kappa-B upon NF-kappa-B the cytoplasm is exerted primarily through the interaction with p65. p65 shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kappa-B complex. Associates with chromatin at the NF-kappa-B promoter region via association with DDX1. Essential for cytokine gene expression in T-cells (PubMed:15790681).

Gene: RELA

Molecular Weight: 65kDa

Gene ID: 5970

UniProt: Q04206

Pathways: NF-kappaB Signaling, RTK Signaling, TCR Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Toll-Like Receptors Cascades, S100 Proteins