NUMB antibody (C-Term)

Catalog No. ABIN6263769

The Rabbit Polyclonal anti-NUMB antibody has been validated for WB, IHC, ELISA, IF and ICC. It is suitable to detect NUMB in samples from Human, Mouse, Rat and Chicken.

Quick Overview for NUMB antibody (C-Term) (ABIN6263769)

Target: NUMB (Numb Homolog (NUMB))

Reactivity: Human, Mouse, Rat, Chicken

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NUMB antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-NUMB Antibody

Binding Specificity: C-Term

Specificity: NUMB Antibody detects endogenous levels of total NUMB.

Predicted Reactivity: Pig,Zebrafish,Bovine,Horse,Sheep,Rabbit,Dog,Chicken

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human NUMB, corresponding to a region within C-terminal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:1000-3000, IF/ICC 1:50-200, IHC 1:50-1:200, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for NUMB

Target: NUMB (Numb Homolog (NUMB))

Alternative Name: NUMB

Background:

Description: Plays a role in the process of neurogenesis. Required throughout embryonic neurogenesis to maintain neural progenitor cells, also called radial glial cells (RGCs), by allowing their daughter cells to choose progenitor over neuronal cell fate. Not required for the proliferation of neural progenitor cells before the onset of neurogenesis. Also involved postnatally in the subventricular zone (SVZ) neurogenesis by regulating SVZ neuroblasts survival and ependymal wall integrity. May also mediate local repair of brain ventricular wall damage.

Gene: NUMB

Molecular Weight: 75 kDa

Gene ID: 8650

UniProt: P49757

Pathways: Cell-Cell Junction Organization