ABCB1 antibody (Internal Region)

Catalog No. ABIN6263928

The Rabbit Polyclonal anti-ABCB1 antibody has been validated for WB, IHC and ELISA. It is suitable to detect ABCB1 in samples from Human, Mouse and Rat. There are 4+ publications available.

Quick Overview for ABCB1 antibody (Internal Region) (ABIN6263928)

Target: ABCB1 (ATP-Binding Cassette, Sub-Family B (MDR/TAP), Member 1 (ABCB1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ABCB1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Product Details anti-ABCB1 Antibody

Binding Specificity: Internal Region

Specificity: P Glycoprotein 1(MDR1) Antibody detects endogenous levels of total P Glycoprotein 1(MDR1).

Predicted Reactivity: Pig,Horse,Rabbit,Dog,Chicken,Xenopus

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human P Glycoprotein 1(MDR1), corresponding to a region within the internal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:1000, IHC 1:50-1:200, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

References for anti-ABCB1 antibody (ABIN6263928)

Shao, Zhang, Ma, Lu, Meng, Li, Wang, Chen, Zhang, Han, Liu, Ma: "MicroRNA-139-5p affects cisplatin sensitivity in human nasopharyngeal carcinoma cells by regulating the epithelial-to-mesenchymal transition." in: Gene, Vol. 652, pp. 48-58, (2018) (PubMed).

Liu, Tang, Liu, Zhang, He, Li, Gao, Tang, Jin, Kong: "Increased autophagy in EOC re-ascites cells can inhibit cell death and promote drug resistance." in: Cell death & disease, Vol. 9, Issue 4, pp. 419, (2018) (PubMed).

Xiang, Ye, Huang, Yu, Chen, Deng, Zhang, Lou, Zhang, Shi, Chen, Zhou: "Brusatol Enhances the Chemotherapy Efficacy of Gemcitabine in Pancreatic Cancer via the Nrf2 Signalling Pathway." in: Oxidative medicine and cellular longevity, Vol. 2018, pp. 2360427, (2018) (PubMed).

Zhou, Lin, Zhang, Zhang, Zhang, Zhang, Xie, Ren: "miR-506 enhances the sensitivity of human colorectal cancer cells to oxaliplatin by suppressing MDR1/P-gp expression." in: Cell proliferation, Vol. 50, Issue 3, (2017) (PubMed).

Target Details for ABCB1

Target: ABCB1 (ATP-Binding Cassette, Sub-Family B (MDR/TAP), Member 1 (ABCB1))

Alternative Name: ABCB1

Background:

Description: Energy-dependent efflux pump responsible for decreased drug accumulation in multidrug-resistant cells.

Gene: ABCB1

Molecular Weight: 141 kDa

Gene ID: 5243

UniProt: P08183