SQSTM1 antibody (C-Term)

Catalog No. ABIN6263985

The Rabbit Polyclonal anti-SQSTM1 antibody has been validated for WB, IHC, ELISA, IF and ICC. It is suitable to detect SQSTM1 in samples from Human, Mouse and Rat. There are 5+ publications available.

Quick Overview for SQSTM1 antibody (C-Term) (ABIN6263985)

Target: SQSTM1 (Sequestosome 1 (SQSTM1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SQSTM1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-SQSTM1 Antibody

Binding Specificity: C-Term

Specificity: SQSTM1/p62 Antibody detects endogenous levels of total SQSTM1/p62.

Predicted Reactivity: Pig,Zebrafish,Bovine,Horse,Sheep,Rabbit,Chicken

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human SQSTM1/p62, corresponding to a region within C-terminal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000, IF/ICC 1:100-1:500, IHC 1:50-1:200, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

References for anti-SQSTM1 antibody (ABIN6263985)

Wang, Mao, Chen: "Xiaoqinglong Decoction Attenuates Chronic Obstructive Pulmonary Disease in Rats via Inhibition of Autophagy." in: Evidence-based complementary and alternative medicine : eCAM, Vol. 2018, pp. 6705871, (2018) (PubMed).

Tang, Cui, Luan, Ruan, Wang, Yang, Wang, Liu, Wang: "Human tissue kallikrein 1 ameliorates erectile function via modulation of macroautophagy in aged transgenic rats." in: Andrology, (2018) (PubMed).

Xia, Zhu, Li, Jin, Zhou, Xiao: "ROS‑mediated autophagy through the AMPK signaling pathway protects INS‑1 cells from human islet amyloid polypeptide‑induced cytotoxicity." in: Molecular medicine reports, Vol. 18, Issue 3, pp. 2744-2752, (2018) (PubMed).

Wu, Zheng, Bai, Xia, Zhang, Guo: "HMGB1/RAGE axis mediates the apoptosis, invasion, autophagy, and angiogenesis of the renal cell carcinoma." in: OncoTargets and therapy, Vol. 11, pp. 4501-4510, (2018) (PubMed).

Wu, Huang, Jiao, Ding, Zhang, Chen, Wang, Li, Huo: "Olaquindox disrupts tight junction integrity and cytoskeleton architecture in mouse Sertoli cells." in: Oncotarget, Vol. 8, Issue 51, pp. 88630-88644, (2017) (PubMed).

Target Details for SQSTM1

Target: SQSTM1 (Sequestosome 1 (SQSTM1))

Alternative Name: SQSTM1

Background:

Description: Autophagy receptor required for selective macroautophagy (aggrephagy). Functions as a bridge between polyubiquitinated cargo and autophagosomes. Interacts directly with both the cargo to become degraded and an autophagy modifier of the MAP1 LC3 family (PubMed:16286508, PubMed:20168092, PubMed:24128730, PubMed:28404643, PubMed:22622177). Along with WDFY3, involved in the formation and autophagic degradation of cytoplasmic ubiquitin-containing inclusions (p62 bodies, ALIS/aggresome-like induced structures). Along with WDFY3, required to recruit ubiquitinated proteins to PML bodies in the nucleus (PubMed:24128730, PubMed:20168092). May regulate the activation of NFKB1 by TNF-alpha, nerve growth factor (NGF) and interleukin-1. May play a role in titin/TTN downstream signaling in muscle cells. May regulate signaling cascades through ubiquitination. Adapter that mediates the interaction between TRAF6 and CYLD (By similarity). May be involved in cell differentiation, apoptosis, immune response and regulation of K+ channels. Involved in endosome organization by retaining vesicles in the perinuclear cloud: following ubiquitination by RNF26, attracts specific vesicle-associated adapters, forming a molecular bridge that restrains cognate vesicles in the perinuclear region and organizes the endosomal pathway for efficient cargo transport (PubMed:27368102).

Gene: SQSTM1

Molecular Weight: 62 kDa

Gene ID: 8878

UniProt: Q13501

Pathways: NF-kappaB Signaling, Neurotrophin Signaling Pathway, Autophagy