Patched 1 antibody (N-Term)

Catalog No. ABIN6264046

This anti-Patched 1 antibody is a Rabbit Polyclonal antibody detecting Patched 1 in WB, ELISA, IHC, IF and ICC. Suitable for Human and Mouse.

Quick Overview for Patched 1 antibody (N-Term) (ABIN6264046)

Target: Patched 1 (PTCH1)

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Patched 1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-Patched 1 Antibody

Binding Specificity: N-Term

Specificity: Patched/PTCH1 Antibody detects endogenous levels of total Patched/PTCH1.

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human Patched/PTCH1, corresponding to a region within N-terminal amino acids.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000, IHC: 1:50-1:200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for Patched 1

Target: Patched 1 (PTCH1)

Alternative Name: PTCH1

Background:

Description: Acts as a receptor for sonic hedgehog (SHH), indian hedgehog (IHH) and desert hedgehog (DHH). Associates with the smoothened protein (SMO) to transduce the hedgehog's proteins signal. Seems to have a tumor suppressor function, as inactivation of this protein is probably a necessary, if not sufficient step for tumorigenesis.

Gene: PTCH1

Molecular Weight: 160kDa

Gene ID: 5727

UniProt: Q13635

Pathways: Hedgehog Signaling, Carbohydrate Homeostasis, Tube Formation