RIPK3 antibody (Internal Region)

Catalog No. ABIN6264768

The Rabbit Polyclonal anti-RIPK3 antibody has been validated for WB, ELISA, ICC and IF. It is suitable to detect RIPK3 in samples from Human and Mouse.

Quick Overview for RIPK3 antibody (Internal Region) (ABIN6264768)

Target: RIPK3 (Receptor-Interacting serine-threonine Kinase 3 (RIPK3))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RIPK3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF)

Product Details anti-RIPK3 Antibody

Binding Specificity: Internal Region

Specificity: RIPK3 Antibody detects endogenous levels of total RIPK3.

Predicted Reactivity: Pig,Bovine,Horse,Sheep

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human RIPK3, corresponding to a region within the internal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:1000-3000, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for RIPK3

Target: RIPK3 (Receptor-Interacting serine-threonine Kinase 3 (RIPK3))

Alternative Name: RIPK3

Background:

Description: Essential for necroptosis, a programmed cell death process in response to death-inducing TNF-alpha family members. Upon induction of necrosis, RIPK3 interacts with, and phosphorylates RIPK1 and MLKL to form a necrosis-inducing complex. RIPK3 binds to and enhances the activity of three metabolic enzymes: GLUL, GLUD1, and PYGL. These metabolic enzymes may eventually stimulate the tricarboxylic acid cycle and oxidative phosphorylation, which could result in enhanced ROS production.

Gene: RIPK3

Molecular Weight: 57 kDa

Gene ID: 11035

UniProt: Q9Y572

Pathways: Activation of Innate immune Response, Toll-Like Receptors Cascades