RIT1 antibody (C-Term)

Catalog No. ABIN6264769

This anti-RIT1 antibody is a Rabbit Polyclonal antibody detecting RIT1 in WB, ELISA, ICC and IF. Suitable for Human, Mouse and Rat.

Quick Overview for RIT1 antibody (C-Term) (ABIN6264769)

Target: RIT1 (Ras-Like Without CAAX 1 (RIT1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RIT1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF)

Product Details anti-RIT1 Antibody

Binding Specificity: C-Term

Specificity: RIT1 Antibody detects endogenous levels of total RIT1.

Predicted Reactivity: Pig,Bovine,Horse,Sheep,Rabbit,Dog,Chicken,Xenopus

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human RIT1, corresponding to a region within C-terminal amino acids.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for RIT1

Target: RIT1 (Ras-Like Without CAAX 1 (RIT1))

Alternative Name: RIT1

Background:

Description: Plays a crucial role in coupling NGF stimulation to the activation of both EPHB2 and MAPK14 signaling pathways and in NGF-dependent neuronal differentiation. Involved in ELK1 transactivation through the Ras-MAPK signaling cascade that mediates a wide variety of cellular functions, including cell proliferation, survival, and differentiation.

Gene: RIT1

Molecular Weight: 25kDa

Gene ID: 6016

UniProt: Q92963

Pathways: Neurotrophin Signaling Pathway