TRIB3 antibody (N-Term)

Catalog No. ABIN6265726

The Rabbit Polyclonal anti-TRIB3 antibody (ABIN6265726) specifically detects TRIB3 in WB and IHC. The antibody is reactive with Human samples.

Quick Overview for TRIB3 antibody (N-Term) (ABIN6265726)

Target: TRIB3 (Tribbles Homolog 3 (Drosophila) (TRIB3))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TRIB3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-TRIB3 Antibody

Binding Specificity: N-Term

Purpose: TRIB3 Antibody

Specificity: TRIB3 Antibody detects endogenous levels of total TRIB3.

Predicted Reactivity: Pig,Bovine,Sheep

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human TRIB3, corresponding to a region within N-terminal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:1000-3000, IHC 1:50-1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for TRIB3

Target: TRIB3 (Tribbles Homolog 3 (Drosophila) (TRIB3))

Alternative Name: TRIB3

Background:

Description: Disrupts insulin signaling by binding directly to Akt kinases and blocking their activation. May bind directly to and mask the 'Thr-308' phosphorylation site in AKT1. Binds to ATF4 and inhibits its transcriptional activation activity. Interacts with the NF-kappa-B transactivator p65 RELA and inhibits its phosphorylation and thus its transcriptional activation activity. Interacts with MAPK kinases and regulates activation of MAP kinases. May play a role in programmed neuronal cell death but does not appear to affect non-neuronal cells. Does not display kinase activity. Inhibits the transcriptional activity of DDIT3/CHOP and is involved in DDIT3/CHOP-dependent cell death during ER stress. Can inhibit APOBEC3A editing of nuclear DNA.

Gene: TRIB3

Molecular Weight: 40 kDa

Gene ID: 57761

UniProt: Q96RU7

Pathways: Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Lipid Metabolism by PPARalpha