CD8 alpha antibody (C-Term, Cytoplasmic Domain)

Catalog No. ABIN6295109

The Mouse Monoclonal anti-CD8 alpha antibody is suitable to detect CD8 alpha in samples from Human. It has been validated for FACS, IHC and IF.

Quick Overview for CD8 alpha antibody (C-Term, Cytoplasmic Domain) (ABIN6295109)

Target: CD8 alpha (CD8A) (CD8a Molecule (CD8A))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD8 alpha antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-CD8 alpha Antibody

Binding Specificity: C-Term, Cytoplasmic Domain

Purpose: Mouse anti-Human CD8 Antibody [Sodium Azide Free]

Specificity: Cell surface

Purification: A 13 amino acid peptide from the C-terminal cytoplasmic domain of the alpha chain was used as immunogen for this CD8 antibody.

Immunogen: A 13 amino acid peptide from the C-terminal cytoplasmic domain of the alpha chain was used as immunogen for this CD8 antibody.

Application Details

Application Notes: Flow Cytometry: 0.5-1 μg/million cells
Immunofluorescence: 0.5-1 μg/mL
Immunohistology (FFPE): 1-2 μg/mL for 30 minutes at RT (1)
Prediluted format: incubate for 30 min at RT (2),

Restrictions: For Research Use only

Handling

Buffer: In 1X PBS ( pH 7.4), BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Target Details for CD8 alpha

Target: CD8 alpha (CD8A) (CD8a Molecule (CD8A))

Alternative Name: T-cell surface glycoprotein CD8 alpha chain

Background:

Target Description: CD8 is a cell surface receptor expressed either as a heterodimer with the beta chain or as a homodimer. A majority of thymocytes and a subpopulation of mature Tcells and NK cells express CD8a. It binds to MHC class 1 and through its association with protein tyrosine kinase p56lck plays a role in Tcell development and activation of mature Tcells. For mature Tcells, CD8 and 4 are mutually exclusive, so antibody to CD8 is generally used in conjunction with antibody to CD4. CD8a antibody is a useful marker for distinguishing helper/inducer T-lymphocytes, and most peripheral Tcell lymphomas are CD4+/CD8-. Anaplastic large cell lymphoma is usually CD4+/CD8-, and in T-lymphoblastic lymphoma/leukemia, they are often co-expressed. It is also found in littoral cell angioma of the spleen.

Gene Symbol: CD8A///MAL

Gene ID: 925

UniProt: P01732

Pathways: TCR Signaling