EpCAM antibody (Extracellular Domain)

Catalog No. ABIN6295463

The Mouse Monoclonal anti-EpCAM antibody is suitable to detect EpCAM in samples from Human. It has been validated for WB, FACS, IHC, IF, ELISA and Coat.

Quick Overview for EpCAM antibody (Extracellular Domain) (ABIN6295463)

Target: EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This EpCAM antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA, Coating (Coat)

Product Details anti-EpCAM Antibody

Binding Specificity: AA 77-202, Extracellular Domain

Purpose: Mouse anti-Human EpCAM Antibody [Sodium Azide Free]

Specificity: Cell surface, cytoplasmic

Purification: A human recombinant partial protein corresponding to amino acids 77-202 (extracellular domain) was used as the immunogen for the Ep-CAM antibody.

Immunogen: A human recombinant partial protein corresponding to amino acids 77-202 (extracellular domain) was used as the immunogen for the Ep-CAM antibody.

Application Details

Application Notes: ELISA: 2-4 μg/mL, order the BSA free formulation for coating
WB: 0.5-1 μg/mL
IF: 1-2 μg/mL
Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT
Flow Cytometry: 0.5-1 μg/million cells in 0.1ml

Restrictions: For Research Use only

Handling

Buffer: In 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Target Details for EpCAM

Target: EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))

Alternative Name: Epithelial cell adhesion molecule

Background:

Target Description: EGP40 is a 40-43 kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas. This antibody has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. This antibody is also useful in distinguishing serous carcinomas of the ovary from mesothelioma.

Gene Symbol: EPCAM///GA733-2///M1S2///M4S1///MIC18///TACSTD1///TROP1

Gene ID: 4072

UniProt: P16422