FBXO5 antibody (N-Term)

Catalog No. ABIN6295467

This anti-FBXO5 antibody is a Mouse Monoclonal antibody detecting FBXO5 in IHC. Suitable for Human.

Quick Overview for FBXO5 antibody (N-Term) (ABIN6295467)

Target: FBXO5 (F-Box Protein 5 (FBXO5))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This FBXO5 antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Product Details anti-FBXO5 Antibody

Binding Specificity: N-Term

Purpose: Mouse anti-Human EMI-1 Antibody [IHC only]

Specificity: Nuclear

Purification: A human partial recombinant N-terminal protein fragment was used as the immunogen for the EMI-1 antibody.

Immunogen: A human partial recombinant N-terminal protein fragment was used as the immunogen for the EMI-1 antibody.

Application Details

Application Notes: Prediluted format : incubate for 30 min at RT (2)

Restrictions: For Research Use only

Handling

Buffer: Prediluted in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide, For IHC use only

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Target Details for FBXO5

Target: FBXO5 (F-Box Protein 5 (FBXO5))

Alternative Name: F-box only protein 5

Background:

Target Description: Early Mitotic Inhibitor-1 regulates mitosis by inhibiting the anaphase promoting complex/cyclosome (APC). It is a conserved F box protein containing a zinc-binding region essential for APC inhibition. The protein functions to promote cyclin A accumulation and S phase entry in somatic cells by inhibiting the APC complex. At the G1-S transition, EMI-1 is transcriptionally induced by the E2F transcription factor. Overexpression accelerates S-phase entry and can override a G1 block caused by overexpression of Cdh1 or the E2F-inhibitor p105 retinoblastoma protein (pRb). Depleting cells of EMI-1 through RNA interference prevents accumulation of cyclin A and inhibits S phase entry.

Gene Symbol: FBXO5///EMI1///FBX5

Gene ID: 26271

UniProt: Q9UKT4

Pathways: Mitotic G1-G1/S Phases