PAX6 antibody (N-Term)

Catalog No. ABIN6295578

This anti-PAX6 antibody is a Mouse Monoclonal antibody detecting PAX6 in IF, FACS and IHC. Suitable for Human.

Quick Overview for PAX6 antibody (N-Term) (ABIN6295578)

Target: PAX6 (Paired Box 6 (PAX6))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This PAX6 antibody is un-conjugated

Application: Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (IHC)

Product Details anti-PAX6 Antibody

Binding Specificity: AA 1-300, N-Term

Purpose: Mouse anti-Human PAX-6 Antibody [Sodium Azide Free]

Specificity: Nuclear

Purification: A recombinant fragment (N-terminus, aa 1-300) was used as the immunogen for the PAX-6 antibody.

Immunogen: A recombinant fragment (N-terminus, aa 1-300) was used as the immunogen for the PAX-6 antibody.

Application Details

Application Notes: Flow Cytometry: 0.5-1 μg/million cells in 0.1ml
Immunofluorescence: 0.5-1 μg/mL
Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1)
Prediluted format: incubate for 30 min at RT (2)

Restrictions: For Research Use only

Handling

Buffer: In 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Target Details for PAX6

Target: PAX6 (Paired Box 6 (PAX6))

Alternative Name: Paired box protein Pax-6

Background:

Target Description: Pax genes contain paired domains with strong homology to genes in Drosophila, which are involved in programming early development. Lesions in the Pax-6 gene account for most cases of aniridia, a congenital malformation of the eye, chiefly characterized by iris hypoplasia, which can cause blindness. Pax-6 is involved in other anterior segment malformations besides aniridia, such as Peters anomaly, a major error in the embryonic development of the eye with corneal clouding with variable iridolenticulocorneal adhesions. The Pax-6 gene encodes a transcriptional regulator that recognizes target genes through its paired-type DNA-binding domain. The paired domain is composed of two distinct DNA-binding subdomains, the amino-terminal subdomain and the carboxy-terminal subdomain, which bind respective consensus DNA sequences. The human Pax-6 gene produces two alternatively spliced isoforms that have the distinct structure of the paired domain.

Gene Symbol: PAX6///AN2

Gene ID: 5080

UniProt: P26367

Pathways: Peptide Hormone Metabolism, Carbohydrate Homeostasis