Cytokeratin 18 antibody

Catalog No. ABIN6295940

The Mouse Monoclonal anti-Cytokeratin 18 antibody is suitable to detect Cytokeratin 18 in samples from Human, Rat, Mouse, Dog, Pig, Cat, Cow, Hamster, Sheep, Horse, Goat, Marmoset and Ferret. It has been validated for WB, IHC, IF and FACS.

Quick Overview for Cytokeratin 18 antibody (ABIN6295940)

Target: Cytokeratin 18 (KRT18) (Keratin 18 (KRT18))

Reactivity: Human, Rat, Mouse, Dog, Pig, Cat, Cow, Hamster, Sheep, Horse, Goat, Marmoset, Ferret

Host: Mouse

Clonality: Monoclonal

Conjugate: This Cytokeratin 18 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Product Details anti-Cytokeratin 18 Antibody

Purpose: Mouse anti-Human/Mouse/Rat/Hamster/Ferret/Cow/Pig/Cat/Dog/Sheep/Goat/Horse/Marmoset Monkey Keratin 18 Antibody [Sodium Azide Free]

Specificity: Cytoplasmic

Purification: A cytoskeletal preparation from A431 cells was used as the immunogen for the Keratin 18 antibody.

Immunogen: A cytoskeletal preparation from A431 cells was used as the immunogen for the Keratin 18 antibody.

Application Details

Application Notes: Western blot: 1-2 μg/mL
Flow Cytometry: 0.5-1 μg/million cells in 0.1ml
Immunofluorescence: 1-2 μg/mL
Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1)
Prediluted format: incubate for 30 min at RT (2)

Restrictions: For Research Use only

Handling

Buffer: In 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Target Details for Cytokeratin 18

Target: Cytokeratin 18 (KRT18) (Keratin 18 (KRT18))

Alternative Name: Keratin, type I cytoskeletal 18

Background:

Target Description: This mAb reacts with a wide variety of simple epithelia. It does not react with stratified squamous epithelia. It reacts with epithelial tumors of the gastrointestinal tract, lung, breast, pancreas, ovary, and thyroid. Cytokeratin 18, which belongs to the type A (acidic) subfamily of low molecular weight keratins, exists in combination with cytokeratin 8. It was reported that tissues from gastrointestinal tract are positive for both cytokeratin 8 and 18 but do not contain cytokeratin 14. Tissues from gastrointestinal tract, respiratory tract and urogenital tract, as well as endocrine and exocrine tissues and mesothelial cells are positive for cytokeratin 18.

Gene Symbol: KRT18///CYK18///PIG46

Gene ID: 3875

UniProt: P05783

Pathways: Apoptosis, Caspase Cascade in Apoptosis