Mitochondria antibody

Catalog No. ABIN6296107

This Mouse Monoclonal antibody specifically detects in IHC, WB and IF. It exhibits reactivity toward Human.

Quick Overview for Mitochondria antibody (ABIN6296107)

Target: Mitochondria

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB), Immunofluorescence (IF)

Product Details

Purpose: Mouse anti-Human Mitochondria Antibody [Sodium Azide Free]

Specificity: Mitochondria in cytoplasm

Purification: A semi-purified mitochondria preparation was used as the immunogen for the Mitochondria antibody.

Immunogen: A semi-purified mitochondria preparation was used as the immunogen for the Mitochondria antibody.

Application Details

Application Notes: Western blot: 1-2 μg/mL
IF: 0.5-1 μg/mL
IHC (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1)
Prediluted format: incubate for 30 min at RT (2)

Restrictions: For Research Use only

Handling

Buffer: In 1X PBS ( pH 7.4), BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Target Details

Target: Mitochondria

Background:

Target Description: Clone MTC02 antibody recognizes a unique 60 kDa antigen that is present only on mitochondria in human cells. It is a part of a panel of reagents which recognizes subcellular organelles or compartments. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. This antibody recognizes an antigen associated with the mitochondria specific to human cells alone. MTC02 mAb can be used to stain the mitochondria in cell or tissue preparations and can be used as a marker in subcellular fractions. The antibody produces a spaghetti-like pattern in normal and malignant cells and may be used to stain mitochondria of cells in fixed or frozen tissue sections. It can also be used with paraformaldehyde fixed frozen tissue or cell preparations. MTC02 antibody is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells, including neurons and embryonic stem cells.