S100B antibody

Catalog No. ABIN6296284

This Rabbit Polyclonal antibody specifically detects S100B in WB, IHC, IF and FACS. It exhibits reactivity toward Human, Rat, Mouse and Cow.

Quick Overview for S100B antibody (ABIN6296284)

Target: S100B (S100 Calcium Binding Protein B (S100B))

Reactivity: Human, Rat, Mouse, Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This S100B antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Product Details anti-S100B Antibody

Purpose: Rabbit anti-Human/Mouse/Rat/Cow Recombinant S100B Antibody [Sodium Azide Free]

Specificity: Cytoplasmic, nuclear

Purification: Full length human recombinant S100B protein was used as the immunogen for this recombinant S100B antibody.

Immunogen: Full length human recombinant S100B protein was used as the immunogen for this recombinant S100B antibody.

Application Details

Application Notes: Flow Cytometry: 0.5-1 μg/million cells in 0.1ml
Immunofluorescence: 1-2 μg/mL
Western blot: 0.5-1 μg/mL
Immunohistochemistry (FFPE): 0.25-0.5 μg/mL for 30 min at RT
Prediluted IHC only format: incubate for 30 min at RT (1)

Restrictions: For Research Use only

Handling

Buffer: In 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Target Details for S100B

Target: S100B (S100 Calcium Binding Protein B (S100B))

Alternative Name: Protein S100-B

Background:

Target Description: S100 belongs to the family of calcium binding proteins. S100A and S100B proteins are two members of the S100 family. S100A is composed of an alpha and a beta chain whereas S100B is composed of two beta chains. This antibody is specific against an epitope located on the beta-chain (i.e. in S-100A and S-100B) but not on the alpha-chain of S-100 (i.e. in S-100A and S100A0). This antibody can be used to localize S-100A and S-100B in various tissue sections. S-100 protein has been found in normal melanocytes, Langerhans cells, histiocytes, chondrocytes, lipocytes, skeletal and cardiac muscle, Schwann cells, epithelial and myoepithelial cells of the breast, salivary and sweat glands, as well as in glial cells. Neoplasms derived from these cells also express S-100 protein, albeit non-uniformly. A large number of well-differentiated tumors of the salivary gland, adipose and cartilaginous tissue, and Schwann cell-derived tumors express S-100 protein. Almost all malignant melanomas and cases of histiocytosis X are positive for S-100 protein.

Gene Symbol: S100B

Gene ID: 6285

UniProt: P04271

Pathways: Regulation of Muscle Cell Differentiation, Positive Regulation of Immune Effector Process, Toll-Like Receptors Cascades, Regulation of long-term Neuronal Synaptic Plasticity, S100 Proteins