MUC1 antibody

Catalog No. ABIN6296461

This Mouse Monoclonal antibody specifically detects MUC1 in IHC, ELISA, FACS, IF and Coat. It exhibits reactivity toward Human and Mouse.

Quick Overview for MUC1 antibody (ABIN6296461)

Target: MUC1 (Mucin 1 (MUC1))

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This MUC1 antibody is un-conjugated

Application: Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS), Immunofluorescence (IF), Coating (Coat)

Product Details anti-MUC1 Antibody

Purpose: Mouse anti-Human/Mouse MUC1 Antibody [Sodium Azide Free]

Specificity: Cytoplasmic and cell surface

Purification: Human milk-fat globule membranes (HMFGM) was used as the immunogen for the MUC1 antibody.

Immunogen: Human milk-fat globule membranes (HMFGM) was used as the immunogen for the MUC1 antibody.

Application Details

Application Notes: ELISA (For coating use Ab at 2-5 μg/mL,order Ab without BSA)
Flow Cytometry: 0.25-0.5 μg/million cells in 0.1ml
Immunofluorescence: 1-2 μg/mL
Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1)
Prediluted format: incubate for 30 min at RT (2)

Restrictions: For Research Use only

Handling

Buffer: In 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Target Details for MUC1

Target: MUC1 (Mucin 1 (MUC1))

Alternative Name: Mucin-1 ]

Background:

Target Description: This mAb reacts with MUC1/Mucin-1/Epithelial Marker Antigen/EMA. The dominant epitope of this mAb has not yet been determined. MUC1 is a large cell surface mucin glycoprotein expressed by most glandular and ductal epithelial cells and some hematopoietic cell lineages. It is expressed on most secretory epithelium, including mammary gland and some hematopoietic cells. It is expressed abundantly in lactating mammary glands and over expressed abundantly in >90 % breast carcinomas and metastases. Transgenic MUC1 has been shown to associate with all four c-erbB receptors and localize with c-erbB1 (EGFR) in lactating glands. The MUC1 gene contains seven exons and produces several different alternatively spliced variants. The major expressed form of MUC1 uses all seven exons and is a type 1 transmembrane protein with a large extracellular tandem repeat domain. The tandem repeat domain is highly O glycosylated and alterations in glycosylation have been shown in epithelial cancer cells. Antibody to EMA is useful as a pan-epithelial marker for detecting early metastatic loci of carcinoma in bone marrow or liver.

Gene Symbol: MUC1///PUM

Gene ID: 4582

UniProt: P15941

Pathways: Negative Regulation of intrinsic apoptotic Signaling