NADPH Oxidase 4 antibody

Catalog No. ABIN6296588

The Mouse Monoclonal anti-NADPH Oxidase 4 antibody has been validated for WB, ELISA, FACS, IF and Coat. It is suitable to detect NADPH Oxidase 4 in samples from Human.

Quick Overview for NADPH Oxidase 4 antibody (ABIN6296588)

Target: NADPH Oxidase 4 (NOX4)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This NADPH Oxidase 4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunofluorescence (IF), Coating (Coat)

Product Details anti-NADPH Oxidase 4 Antibody

Purpose: Mouse anti-Human NOX4 Antibody [Sodium Azide Free]

Specificity: Cytoplasmic and cell surface

Purification: Human recombinant protein was used as the immunogen for this NOX4 antibody.

Immunogen: Human recombinant protein was used as the immunogen for this NOX4 antibody.

Application Details

Application Notes: FACS: 0.5-1 μg/10^6 cells in 0.1ml
IF: 1-2 μg/mL,ELISA: suitable, order BSA-free formulation for coating
WB: 0.5-1 μg/mL

Restrictions: For Research Use only

Handling

Buffer: In 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Target Details for NADPH Oxidase 4

Target: NADPH Oxidase 4 (NOX4)

Alternative Name: NADPH oxidase 4

Background:

Target Description: The superoxide-generating NADPH oxidase includes a membrane-bound flavocytochrome containing two subunits, gp91-phox and p22-phox, and the cytosolic proteins p47-phox and p67-phox. During activation of the NADPH oxidase, p47-phox and p67-phox migrate to the plasma membrane where they associate with the flavocytochrome, cytochrome b558, to form the active enzyme complex. The p22 and gp91-phox subunits also function as surface O2 sensors that initiate cellular signaling in response to hypoxic conditions. NOX4 is a renal gp91-phox homolog highly expressed at the site of erythropoietin production in the proximal convoluted tubule epithelial cells of the renal cortex. It is also expressed in fetal tissues, placenta, glioblastoma and vascular cells.

Gene Symbol: NOX4///RENOX

Gene ID: 4821

UniProt: Q9NPH5

Pathways: Carbohydrate Homeostasis, Smooth Muscle Cell Migration