Vimentin antibody

Catalog No. ABIN6296593

This Mouse Monoclonal antibody specifically detects Vimentin in WB, IHC, IF and FACS. It exhibits reactivity toward Human, Pig, Cow, Chicken, Dog, Cat and Goat.

Quick Overview for Vimentin antibody (ABIN6296593)

Target: Vimentin (VIM)

Reactivity: Human, Pig, Cow, Chicken, Dog, Cat, Goat

Host: Mouse

Clonality: Monoclonal

Conjugate: This Vimentin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Product Details anti-Vimentin Antibody

Purpose: Mouse anti-Human/Cow/Dog/Cat/Pig/Goat/Chicken VIM Antibody [Sodium Azide Free]

Specificity: Cytoplasmic

Purification: Human recombinant protein was used as the immunogen for this VIM antibody.

Immunogen: Human recombinant protein was used as the immunogen for this VIM antibody.

Application Details

Application Notes: Flow Cytometry: 0.5-1 μg/million cells in 0.1ml
Immunofluorescence: 1-2 μg/mL
Western blot: 0.25-0.5 μg/mL
Immunohistochemistry (FFPE): 0.25-0.5 μg/mL for 30 min at RT (1)
Prediluted format: incubate for 30 min at RT (2)

Restrictions: For Research Use only

Handling

Buffer: In 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Target Details for Vimentin

Target: Vimentin (VIM)

Alternative Name: Vimentin

Background:

Target Description: This mAb reacts with a 58 kDa protein identified as vimentin. It shows no cross-reaction with other closely related intermediate filament proteins (IFPs) such as desmin, keratin, neurofilament, and glial fibrillary acid protein. Anti-vimentin alone is of limited value as a diagnostic tool, however, when used in panels with other antibodies, it is useful for the sub-classification of a given tumor. Expression of vimentin, when used in conjunction with anti-keratin, is helpful when distinguishing melanomas from undifferentiated carcinomas and large cell lymphomas. All melanomas and Schwannomas react strongly with anti-vimentin. It labels a variety of mesenchymal cells, including melanocytes, lymphocytes, endothelial cells, and fibroblasts. Non-reactivity of anti-vimentin is often considered more useful than its positive reactivity, since there are a few tumors that do not contain vimentin, e.g. hepatoma and seminoma. Anti-vimentin is also useful as a tissue process control reagent.

Gene Symbol: VIM

Gene ID: 7431

UniProt: P08670

Pathways: Caspase Cascade in Apoptosis