Melanoma gp100 antibody

Catalog No. ABIN6296696

The Mouse Monoclonal anti-Melanoma gp100 antibody has been validated for IHC, IF and FACS. It is suitable to detect Melanoma gp100 in samples from Human and Horse.

Quick Overview for Melanoma gp100 antibody (ABIN6296696)

Target: Melanoma gp100 (PMEL) (Premelanosome Protein (PMEL))

Reactivity: Human, Horse

Host: Mouse

Clonality: Monoclonal

Conjugate: This Melanoma gp100 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Product Details anti-Melanoma gp100 Antibody

Purpose: Mouse anti-Human/Horse gp100 Antibody [Sodium Azide Free]

Specificity: Cytoplasmic

Purification: Membranes from a human melanoma metastasis were used as the immunogen for this anti-gp100 antibody.

Immunogen: Membranes from a human melanoma metastasis were used as the immunogen for this anti-gp100 antibody.

Application Details

Application Notes: Flow Cytometry: 0.5-1 μg/million cells in 0.1ml
Immunofluorescence: 1-2 μg/mL
Immunohistochemistry (FFPE): 1-2 μg/mL for 30 minutes at RT (1)

Restrictions: For Research Use only

Handling

Buffer: In 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Target Details for Melanoma gp100

Target: Melanoma gp100 (PMEL) (Premelanosome Protein (PMEL))

Alternative Name: Melanocyte protein PMEL , M-beta]

Background:

Target Description: By immunohistochemistry, it specifically recognizes a protein in melanocytes and melanomas. This mAb reacts with junctional and blue nevus cells and variably with fetal and neonatal melanocytes. Intradermal nevi, normal adult melanocytes, and non-melanocytic cells are negative. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin.This Mab labels formalin-fixed, paraffin-embedded melanomas and other tumors showing melanocytic differentiation.

Gene Symbol: PMEL///D12S53E///PMEL17///SILV

Gene ID: 6490

UniProt: P40967