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CD176 antibody

CD176 Reactivity: Human, Mouse, Rat IHC, IF Host: Mouse Monoclonal unconjugated
Catalog No. ABIN6296729
  • Target See all CD176 products
    CD176 (Thomsen Friedenreich Antigen (CD176))
    Reactivity
    Human, Mouse, Rat
    Host
    • 4
    Mouse
    Clonality
    • 4
    Monoclonal
    Conjugate
    • 4
    This CD176 antibody is un-conjugated
    Application
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Immunohistochemistry (IHC), Immunofluorescence (IF)
    Purpose
    Mouse anti-Human/Mouse/Rat Thomsen-Friedenreich Antigen Antibody [Sodium Azide Free]
    Specificity
    Cell surface
    Purification
    Neuraminidase-treated human red blood cells were used as the immunogen for the Thomsen-Friedenreich Antigen antibody.
    Immunogen
    Neuraminidase-treated human red blood cells were used as the immunogen for the Thomsen-Friedenreich Antigen antibody.
  • Application Notes
    Immunofluorescence: 0.5-1 μg/mL
    Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT
    Restrictions
    For Research Use only
  • Buffer
    In 1X PBS, BSA free, sodium azide free
    Preservative
    Azide free
    Storage
    4 °C,-20 °C
    Storage Comment
    2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.
  • Target
    CD176 (Thomsen Friedenreich Antigen (CD176))
    Alternative Name
    Thomsen-Friedenreich Antigen (CD176 Products)
    Background

    Target Description: Recognizes a disaccharide epitope, Gal1-3GalNAc, of Thomsen-Friedenreich (TF) antigen. It is specific for both anomeric forms of the disaccharide (TF and TF, including related structures on the glycolipid) and shows no cross-reactivity with sialylated glycophorin. The Thomsen-Friedenreich antigen acts as an oncofetal antigen, with low expression in normal adult tissues but increasing to fetal levels of expression in hyperplasia or malignancy. It is considered as a pan-carcinoma marker. During metastasis, the ability of malignant cells to form multicellular aggregates via homotypic or heterotypic aggregation and their adhesion to the endothelium are critical. The tumor-associated carbohydrate Thomsen-Friedenreich antigen (Gal-GalNAc) is involved in tumor cell adhesion and tissue invasion. It also causes an immune response, and overexpression of the antigen causes cancer cells to be more sensitive to natural killer cell lysis. The Thomsen-Friedenreich antigen is suppressed in normal healthy cells and represents one of the few chemically well-defined antigens associated with tumor malignancy. The presence of the Thomsen-Friedenreich antigen on the surface of cancer cells may result from a divergence from the normal pathway for O-linked glycosylation in these cells, most likely caused by inappropriate localization of the enzymes involved in synthesis of the disaccharide.

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