CD176 antibody

Catalog No. ABIN7862342

This Mouse Monoclonal antibody specifically detects CD176 in IF. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for CD176 antibody (ABIN7862342)

Target: CD176 (Thomsen Friedenreich Antigen (CD176))

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD176 antibody is un-conjugated

Application: Immunofluorescence (IF)

Clone: A78-G-A7

Product Details anti-CD176 Antibody

Purpose: Thomsen-Friedenreich Antigen / CD176 (Pan Carcinoma Marker) Antibody

Specificity: Recognizes a disaccharide epitope, Gal 79oΩ 79oΩ1-3GalNAc, of Thomsen-Friedenreich (TF) antigen. It is specific for both anomeric forms of the disaccharide (TF and TF 79oΩ 79oΩ, including related structures on the glycolipid) and shows no cross-reactivity with sialylated glycophorin. The Thomsen-Friedenreich antigen acts as an oncofetal antigen, with low expression in normal adult tissues but increasing to fetal levels of expression in hyperplasia or malignancy. It is considered as a pan-carcinoma marker. This MAb is capable to agglutinate desialylated red blood cells. During metastasis, the ability of malignant cells to form multicellular aggregates via homotypic or heterotypic aggregation and their adhesion to the endothelium are critical. The tumor-associated carbohydrate Thomsen-Friedenreich antigen (Gal-GalNAc) is involved in tumor cell adhesion and tissue invasion. It also causes an immune response, and overexpression of the antigen causes cancer cells to be more sensitive to natural killer cell lysis. The Thomsen-Friedenreich antigen is suppressed in normal healthy cells and represents one of the few chemically well-defined antigens associated with tumor malignancy. The presence of the Thomsen-Friedenreich antigen on the surface of cancer cells may result from a divergence from the normal pathway for O-linked glycosylation in these cells, most likely caused by inappropriate localization of the enzymes involved in synthesis of the disaccharide.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate.

Immunogen: Neuraminidase-treated human red blood cells

Isotype: IgM, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for CD176

Target: CD176 (Thomsen Friedenreich Antigen (CD176))

Alternative Name: Thomsen-Friedenreich Antigen

Background:

T-F Antigen, TF Antigen, Asialoglycophorin, pan-carcinoma marker, CD176

KG1 cells or human colorectal carcinoma tissues.