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CD176 antibody

CD176 Reactivity: Human, Mouse, Rat IHC, IF, StM Host: Mouse Monoclonal A78-G-A7 unconjugated
Catalog No. ABIN6941356
  • Target See all CD176 products
    CD176 (Thomsen Friedenreich Antigen (CD176))
    Reactivity
    Human, Mouse, Rat
    Host
    • 4
    Mouse
    Clonality
    • 4
    Monoclonal
    Conjugate
    • 4
    This CD176 antibody is un-conjugated
    Application
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    Immunohistochemistry (IHC), Immunofluorescence (IF), Staining Methods (StM)
    Specificity
    Recognizes a disaccharide epitope, Gal 1-3GalNAc, of Thomsen-Friedenreich (TF) antigen. It is specific for both anomeric forms of the disaccharide (TF and TF , including related structures on the glycolipid) and shows no cross-reactivity with sialylated glycophorin. The Thomsen-Friedenreich antigen acts as an oncofetal antigen, with low expression in normal adult tissues but increasing to fetal levels of expression in hyperplasia or malignancy. It is considered as a pan-carcinoma marker. This MAb is capable to agglutinate desialylated red blood cells. During metastasis, the ability of malignant cells to form multicellular aggregates via homotypic or heterotypic aggregation and their adhesion to the endothelium are critical. The tumor-associated carbohydrate Thomsen-Friedenreich antigen (Gal-GalNAc) is involved in tumor cell adhesion and tissue invasion. It also causes an immune response, and overexpression of the antigen causes cancer cells to be more sensitive to natural killer cell lysis. The Thomsen-Friedenreich antigen is suppressed in normal healthy cells and represents one of the few chemically well-defined antigens associated with tumor malignancy. The presence of the Thomsen-Friedenreich antigen on the surface of cancer cells may result from a divergence from the normal pathway for O-linked glycosylation in these cells, most likely caused by inappropriate localization of the enzymes involved in synthesis of the disaccharide.
    Immunogen
    Neuraminidase-treated human red blood cells
    Clone
    A78-G-A7
    Isotype
    IgM kappa
  • Application Notes

    Positive Control: KG1 cells. Human colorectal carcinoma tissues.

    Known Application: Immunofluorescence (0.5-1 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1.0 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

    Restrictions
    For Research Use only
  • Concentration
    200 μg/mL
    Buffer
    10 mM PBS with 0.05 % BSA & 0.05 % azide.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C,-80 °C
    Storage Comment
    Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
    Expiry Date
    24 months
  • Target
    CD176 (Thomsen Friedenreich Antigen (CD176))
    Alternative Name
    Thomsen-Friedenreich Antigen / CD176 (Pan Carcinoma Marker) (CD176 Products)
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