RNMT antibody

Catalog No. ABIN6296897

The Mouse Monoclonal anti-RNMT antibody has been validated for WB, IHC, IF and FACS. It is suitable to detect RNMT in samples from Human, Rat, Cow and Dog.

Quick Overview for RNMT antibody (ABIN6296897)

Target: RNMT (RNA Guanine-7 Methyltransferase (RNMT))

Reactivity: Human, Rat, Cow, Dog

Host: Mouse

Clonality: Monoclonal

Conjugate: This RNMT antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Product Details anti-RNMT Antibody

Purpose: Mouse anti-Human/Cow/Dog/Rat TAG-72 Antibody [Sodium Azide Free]

Specificity: Cytoplasmic and cell surface

Purification: Purified TAG-72 protein was used as the immunogen for this TAG-72 antibody.

Immunogen: Purified TAG-72 protein was used as the immunogen for this TAG-72 antibody.

Application Details

Application Notes: Western blot: 1-2 μg/mL
Flow Cytometry: 0.5-1 μg/million cells in 0.1ml
Immunofluorescence: 1-2 μg/mL
Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1)
Prediluted format: incubate for 30 min at RT (2)

Restrictions: For Research Use only

Handling

Buffer: In 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Target Details for RNMT

Target: RNMT (RNA Guanine-7 Methyltransferase (RNMT))

Alternative Name: mRNA cap guanine-N7 methyltransferase  

Background:

Target Description: Recognizes an oncofetal antigen of 220 kDa, identified as a tumor-associated glycoprotein (TAG-72) with properties of a mucin. This mAb defines the mucin-carried sialylated-Tn epitope. TAG-72 is usually expressed by adenocarcinomas, but is negative in mesotheliomas. Studies have reported that this antibody has 80 % sensitivity and 93 % specificity for pulmonary adenocarcinoma. Therefore, TAG-72 is a useful marker to distinguish between mesothelioma and adenocarcinoma. However, false positive reactions can occur so results must be interpreted with the utmost caution. This antibody may be useful in the differentiation of non-small cell carcinomas from small cell carcinomas of the lung. The combined use of anti-TAG-72 and anti-GCDFP-15 is valuable in the diagnosis of apocrine carcinoma.

Gene Symbol: tag-72  C25A1.3

Gene ID: 182875

UniProt: Q9XVS1