Insulin antibody

Catalog No. ABIN6297596

The Mouse Monoclonal anti-Insulin antibody has been validated for IHC and IF. It is suitable to detect Insulin in samples from Human, Mouse and Rat.

Quick Overview for Insulin antibody (ABIN6297596)

Target: Insulin (INS)

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This Insulin antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-Insulin Antibody

Purpose: Mouse anti-Human/Mouse/Rat Insulin Antibody [Sodium Azide Free]

Specificity: Cytoplasmic

Purification: Recombinant INS protein was used as the immunogen for the Insulin antibody.

Immunogen: Recombinant INS protein was used as the immunogen for the Insulin antibody.

Application Details

Application Notes: Immunofluorescence: 1-2 μg/mL
Immunohistochemistry (FFPE): 0.1-0.2 μg/mL for 30 min at RT (1)
Prediluted format: incubate for 30 min at RT (2)

Restrictions: For Research Use only

Handling

Buffer: In 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Target Details for Insulin

Target: Insulin (INS)

Alternative Name: Insulin

Background:

Target Description: After removal of the precursor signal peptide, proinsulin is post-translationally cleaved into three peptides: the B chain and A chain peptides, which are covalently linked via two disulfide bonds to form insulin, and C-peptide. Binding of insulin to the insulin receptor (INSR) stimulates glucose uptake. A multitude of mutant alleles with phenotypic effects have been identified. [RefSeq]

Gene Symbol: INS

Gene ID: 3630

UniProt: P01308

Pathways: NF-kappaB Signaling, RTK Signaling, Positive Regulation of Peptide Hormone Secretion, Peptide Hormone Metabolism, Hormone Activity, Carbohydrate Homeostasis, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Autophagy, Negative Regulation of intrinsic apoptotic Signaling, Brown Fat Cell Differentiation, Positive Regulation of fat Cell Differentiation