Cytokeratin 1 antibody

Catalog No. ABIN6297651

This anti-Cytokeratin 1 antibody is a Mouse Monoclonal antibody detecting Cytokeratin 1 in WB, IHC, FACS and IF. Suitable for Human, Rat, Mouse, Cow, Rabbit, Chicken, Monkey and Dog.

Quick Overview for Cytokeratin 1 antibody (ABIN6297651)

Target: Cytokeratin 1 (KRT1) (Keratin 1 (KRT1))

Reactivity: Human, Rat, Mouse, Cow, Rabbit, Chicken, Monkey, Dog

Host: Mouse

Clonality: Monoclonal

Conjugate: This Cytokeratin 1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF)

Product Details anti-Cytokeratin 1 Antibody

Purpose: Mouse anti-Human/Monkey/Cow/Dog/Rabbit/Mouse/Rat/Chicken Basic Cytokeratin Antibody [Sodium Azide Free]

Specificity: Cytoplasmic

Purification: Solubilized cytokeratin extract from human stratum corneum was used as the immunogen.

Immunogen: Solubilized cytokeratin extract from human stratum corneum was used as the immunogen.

Application Details

Application Notes: FACS: 0.5-1 μg/million cells
IF: 1-2 μg/mL
WB: 0.5-1 μg/mL
IHC (FFPE): 0.5-1 μg/mL for 30 min at RT (1)
Prediluted format: incubate for 30 min at RT (2)

Restrictions: For Research Use only

Handling

Buffer: In 1X PBS ( pH 7.4), BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Target Details for Cytokeratin 1

Target: Cytokeratin 1 (KRT1) (Keratin 1 (KRT1))

Alternative Name: Keratin, type II cytoskeletal 1

Background:

Target Description: Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI <5.7) and basic (pI >6.0) subfamilies. This antibody recognizes basic (Type II or HMW) cytokeratins, which include 67 kDa (CK1), 64 kDa (CK3), 59 kDa (CK4), 58 kDa (CK5), 56 kDa (CK6), 52 kDa (CK8). Many studies have shown the usefulness of keratin markers in cancer research and tumor diagnosis. The basic family clone AE3 is commonly used in combination with clone AE1 as a pan cytokeratin antibody acidic + basic cocktail (< a href=../pan-cytokeratin-antibody-cocktail-ae1-ae3-v2330>Cat No V2330).

Gene Symbol: KRT1///KRTA

Gene ID: 3848

UniProt: P04264