Cytokeratin 2 antibody

Catalog No. ABIN6297654

Product Details anti-Cytokeratin 2 Antibody

Target: Cytokeratin 2 (KRT76) (Cytokeratin-2P (KRT76))

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This Cytokeratin 2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Purpose: Mouse anti-Human/Mouse/Rat Basic Cytokeratin Antibody [Sodium Azide Free]

Specificity: Cytoplasmic

Purification: Solubilized keratin extract from human stratum corneum was used as the immunogen for the Basic Cytokeratin antibody.

Immunogen: Solubilized keratin extract from human stratum corneum was used as the immunogen for the Basic Cytokeratin antibody.

Application Details

Application Notes: Flow Cytometry: 0.5-1 μg/million cells in 0.1ml
Immunofluorescence: 0.5-1 μg/mL
Western blot: 0.5-1 μg/mL
Immunohistochemistry (FFPE): 0.25-0.5 μg/mL for 30 min at RT (1)
Prediluted format: incubate for 30 min at RT (2)

Restrictions: For Research Use only

Handling

Buffer: In 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Target Details for Cytokeratin 2

Target: Cytokeratin 2 (KRT76) (Cytokeratin-2P (KRT76))

Alternative Name: Keratin, type II cytoskeletal 2 oral (KRT76 Products)

Synonyms: HUMCYT2A antibody, KRT2B antibody, KRT2P antibody, 2310001L23Rik antibody, keratin 76 antibody, KRT76 antibody, Krt76 antibody

Background:

Target Description: This antibody recognizes CK1, CK5, CK10 and CK14. In normal epithelia, it stains stratified epithelia, myoepithelial cells and basal cells in the prostate gland and bronchi. This mAb shows no reactivity with hepatocytes, pancreatic acinar cells, proximal renal tubules, or endometrial glands, there is no reactivity with cells derived from simple epithelia. Mesenchymal tumors, lymphomas, melanomas, neural tumors, and neuroendocrine tumors are negative with this antibody. It stains myoepithelial cells and has been shown to be useful in distinguishing prostate adenocarcinoma from benign prostate. This antibody has also been useful in separating benign from malignant intraductal breast proliferations.

Gene Symbol: KRT76///KRT2B///KRT2P

Gene ID: 51350

UniProt: Q01546