ICAM-3/CD50 antibody

Catalog No. ABIN6297675

Product Details anti-ICAM-3/CD50 Antibody

Target: ICAM-3/CD50 (ICAM3) (Intercellular Adhesion Molecule 3 (ICAM3))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This ICAM-3/CD50 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Functional Studies (Func)

Purpose: Mouse anti-Human ICAM-3 Antibody [Sodium Azide Free]

Specificity: Cell surface

Purification: Stimulated human leukocytes were used as the immunogen for the anti-ICAM-3 antibody.

Immunogen: Stimulated human leukocytes were used as the immunogen for the anti-ICAM-3 antibody.

Application Details

Application Notes: Functional Activity: Blocks binding of CD11a (LFA-1) to CD50 (ICAM-3) (Order SAF formulation)
Flow Cytometry: 0.5-1 μg/million cells in 0.1ml
Immunofluorescence: 0.5-1 μg/mL
Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1)
Prediluted format: incubate for 30 min at RT (2)

Restrictions: For Research Use only

Handling

Buffer: In 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Target Details for ICAM-3/CD50

Target: ICAM-3/CD50 (ICAM3) (Intercellular Adhesion Molecule 3 (ICAM3))

Alternative Name: Intercellular adhesion molecule 3 (ICAM3 Products)

Synonyms: CD50 antibody, CDW50 antibody, ICAM-R antibody, ICAM-3 antibody, ICAM3 antibody, intercellular adhesion molecule 3 antibody, ICAM3 antibody, Icam3 antibody

Background:

Target Description: Recognizes the D1 domain of an N-glycosylated glycoprotein of 120 kDa with intra-chain disulfide bonds, identified as CD50 or ICAM-3. CD50 is the major ligand for LFA-1 (CD11a/CD18) and may have signaling role to increase adhesion. It is expressed on thymocytes and T lymphocytes and is resistant to treatment with phosphatidylinositol (PI) phospholipase C. This mAb is excellent for staining of formalin/paraffin tissues.

Gene Symbol: ICAM3

Gene ID: 3385

UniProt: P32942