CD44 antibody

Catalog No. ABIN6297699

This anti-CD44 antibody is a Mouse Monoclonal antibody detecting CD44 in FACS, WB, IHC, ELISA, IF and Coat. Suitable for Human, Baboon and Green Monkey.

Quick Overview for CD44 antibody (ABIN6297699)

Target: CD44

Reactivity: Human, Baboon, Green Monkey

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD44 antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Coating (Coat)

Product Details anti-CD44 Antibody

Purpose: Mouse anti-Human/Baboon/Green Monkey HCAM Antibody CD44 [Sodium Azide Free]

Specificity: Cell surface

Purification: Stimulated human leukocytes were used as the immunogen for this CD44 / HCAM antibody.

Immunogen: Stimulated human leukocytes were used as the immunogen for this CD44 / HCAM antibody.

Application Details

Application Notes: ELISA: order BSA-free format for coating
FACS: 0.5-1 μg/million cells
IF: 0.5-1 μg/mL
WB: 0.5-1 μg/mL
IHC (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1)
Prediluted format: incubate for 30 min at RT (2)

Restrictions: For Research Use only

Handling

Buffer: In 1X PBS ( pH 7.4), BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Target Details for CD44

Target: CD44

Alternative Name: CD44 antigen

Background:

Target Description: This antibody recognizes a cell surface glycoprotein of 80-95 kDa, called CD44, or HCAM, on lymphocytes, monocytes, and granulocytes (Leucocyte Typing Workshop V). Its epitope is resistant to digestion by trypsin and chymotrypsin. The CD44 family of glycoproteins exists in a number of variant isoforms, the most common being the standard 85-95 kDa or hematopoietic variant (CD44-s). Higher molecular weight isoforms are described in epithelial cells (CD44-v), which are believed to function in intercellular adhesion and stromal binding. HCAM antibody immunostaining is commonly used for the discrimination of urothelial transitional cell carcinoma in-situ from non-neoplastic changes in the urothelium.

Gene Symbol: CD44///LHR///MDU2///MDU3///MIC4

Gene ID: 960

UniProt: P16070

Pathways: Glycosaminoglycan Metabolic Process, Autophagy, Negative Regulation of intrinsic apoptotic Signaling