IGKV1D-16 antibody

Catalog No. ABIN6297706

This Mouse Monoclonal antibody specifically detects IGKV1D-16 in IHC, FACS and WB. It exhibits reactivity toward Human.

Quick Overview for IGKV1D-16 antibody (ABIN6297706)

Target: IGKV1D-16 (Immunoglobulin kappa Variable 1D-16 (IGKV1D-16))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This IGKV1D-16 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Flow Cytometry (FACS), Western Blotting (WB)

Product Details anti-IGKV1D-16 Antibody

Purpose: Mouse anti-Human Kappa Light Chain Antibody [Sodium Azide Free]

Specificity: Cell surface, cytoplasmic and secreted

Purification: Stimulated human lymphocytes were used as the immunogen for the Kappa Light Chain antibody.

Immunogen: Stimulated human lymphocytes were used as the immunogen for the Kappa Light Chain antibody.

Application Details

Application Notes: Flow Cytometry: 0.5-2.0 μg/million cells in 0.1ml
Western blot: 0.5-1 μg/mL
Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1)
Prediluted format: incubate for 30 min at RT (2)

Restrictions: For Research Use only

Handling

Buffer: In 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Target Details for IGKV1D-16

Target: IGKV1D-16 (Immunoglobulin kappa Variable 1D-16 (IGKV1D-16))

Alternative Name: Immunoglobulin kappa variable 1D-16

Background:

Target Description: This mAb is specific to kappa light chain of immunoglobulin and shows no cross-reaction with lambda light chain or any of the five heavy chains. In mammals, the two light chains in an antibody are always identical, with only one type of light chain, kappa or lambda. The ratio of Kappa to Lambda is 70:30. However, with the occurrence of multiple myeloma or other B-cell malignancies this ratio is disturbed. Antibody to the kappa light chain is reportedly useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin's lymphomas. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is malignant.

Gene Symbol: IGKV1D-16///IGKC

Gene ID: 3514

UniProt: P01601, P01834