CD74 antibody

Catalog No. ABIN6297713

The Mouse Monoclonal anti-CD74 antibody has been validated for IHC. It is suitable to detect CD74 in samples from Human.

Quick Overview for CD74 antibody (ABIN6297713)

Target: CD74 (HLA-DR-gamma (CD74))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD74 antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Product Details anti-CD74 Antibody

Purpose: Mouse anti-Human CD74 Antibody Cocktail [IHC only]

Specificity: Cell surface and paranuclear globular

Purification: SU-DHL-4 lymphoma cells (LN-2) and recombinant full-length human protein (CLIP/813) were used as the immunogen for the CD74 antibody cocktail.

Immunogen: SU-DHL-4 lymphoma cells (LN-2) and recombinant full-length human protein (CLIP/813) were used as the immunogen for the CD74 antibody cocktail.

Application Details

Application Notes: Prediluted format : incubate for 30 min at RT (2)

Restrictions: For Research Use only

Handling

Buffer: Prediluted in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide, For IHC use only

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Target Details for CD74

Target: CD74 (HLA-DR-gamma (CD74))

Alternative Name: HLA class II histocompatibility antigen gamma chain

Background:

Target Description: It recognizes a protein of ~35 kDa, identified as CD74/CLIP. It is a type II transmembrane protein which binds to the peptide binding groove of newly synthesized MHC class II alpha/beta heterodimers and prevents their premature association with endogenous polypeptides. CD74 is expressed primarily by antigen presenting cells, such as B-lymphocytes (from before the pre-B cell stage to before the plasma cell stage), macrophages, and monocytes, and many epithelial cells. Anti-CD74 stains predominantly germinal center lymphocytes and B-cell lymphomas, but rarely T-cell lymphomas. Anti-CD74 has been shown to be useful in differentiating atypical fibroxanthoma (-) from malignant fibrous histiocytoma (+).

Gene Symbol: CD74///DHLAG

Gene ID: 296

UniProt: P04233

Pathways: Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Negative Regulation of intrinsic apoptotic Signaling, Cancer Immune Checkpoints