CD74 antibody

Catalog No. ABIN6297729

This anti-CD74 antibody is a Mouse Monoclonal antibody detecting CD74 in FACS, WB and IF. Suitable for Human.

Quick Overview for CD74 antibody (ABIN6297729)

Target: CD74 (HLA-DR-gamma (CD74))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD74 antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunofluorescence (IF)

Product Details anti-CD74 Antibody

Purpose: Mouse anti-Human CD74 Antibody [Sodium Azide Free]

Specificity: Cell surface and paranuclear globular

Purification: The B lymphoblastoid cell line HFB1 was used as the immunogen for this antibody.

Immunogen: The B lymphoblastoid cell line HFB1 was used as the immunogen for this antibody.

Application Details

Application Notes: FACS: 0.5-1 μg/million cells
IF: 1-2 μg/mL
WB: 0.5-1 μg/mL

Restrictions: For Research Use only

Handling

Buffer: In 1X PBS ( pH 7.4), BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Target Details for CD74

Target: CD74 (HLA-DR-gamma (CD74))

Alternative Name: HLA class II histocompatibility antigen gamma chain

Background:

Target Description: This antibody recognizes proteins of 33, 35 and 41 kDa, which are identified as various isoforms of CD74. The CD74 antibody epitope is localized in the extracellular domain of CD74. CD74 is a type II transmembrane protein which binds to the peptide binding groove of newly synthesized MHC class II alpha/beta heterodimers and prevents their premature association with endogenous polypeptides. The CD74 Molecule plays a critical role in the presentation of peptides, by the MHC class II antigens, to CD4 positive lymphocytes. CD74 is expressed on MHC class II positive cells including B cells, a subset of activated T cells, monocytes, dendritic cells and by various types of carcinomas. CD74 is expressed primarily by antigen presenting cells, such as B-lymphocytes (from before the pre-B cell stage to before the plasma cell stage), macrophages, and monocytes, and many epithelial cells. CD74 antibody stains predominantly germinal center lymphocytes and B-cell lymphomas, but rarely T-cell lymphomas. CD74 antibody has been shown to be useful in differentiating atypical fibroxanthoma (-) from malignant fibrous histiocytoma (+).

Gene Symbol: CD74///DHLAG

Gene ID: 972

UniProt: P04233

Pathways: Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Negative Regulation of intrinsic apoptotic Signaling, Cancer Immune Checkpoints