EGFR antibody

Catalog No. ABIN6297747

The Mouse Monoclonal anti-EGFR antibody (ABIN6297747) specifically detects EGFR in IF, FACS and IHC (fp). The antibody is reactive with Human samples.

Quick Overview for EGFR antibody (ABIN6297747)

Target: EGFR (Epidermal Growth Factor Receptor (EGFR))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This EGFR antibody is un-conjugated

Application: Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Product Details anti-EGFR Antibody

Purpose: Mouse anti-Human EGFR Antibody [Sodium Azide Free]

Specificity: Cell surface

Purification: The immunizing protein for this antibody was recombinant human EGFR.

Immunogen: The immunizing protein for this antibody was recombinant human EGFR.

Application Details

Application Notes: IHC (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1)
FACS: 0.5-1 μg/million cells in 0.1ml
IF: 1-2 μg/mL

Restrictions: For Research Use only

Handling

Buffer: In 1X PBS ( pH 7.4), BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Target Details for EGFR

Target: EGFR (Epidermal Growth Factor Receptor (EGFR))

Alternative Name: Epidermal growth factor receptor

Background:

Target Description: This antibody recognizes a protein of 170 kDa, identified as EGFR.  < a href=../search_result.php?search_txt=egfr>EGFR  is type I receptor tyrosine kinase with sequence homology to  < a href=../search_result.php?search_txt=erbb2>ErbB2, -3 -4 or  < a href=../search_result.php?search_txt=her2>HER2, -3 -4. It binds to Epidermal Growth Factor (EGF),  < a href=../search_result.php?search_txt=TGFa>Transforming Growth Factor-a  (TGF-a), Heparin-binding EGF (HB-EGF), amphiregulin, betacellulin and epiregulin. EGFR is overexpressed in tumors of breast, brain, bladder, lung, gastric, head/neck, esophagus, cervix, vulva, ovary, and endometrium. It is predominantly present in squamous cell carcinomas.

Gene Symbol: EGFR///ERBB///ERBB1///HER1

Gene ID: 1956

UniProt: P00533

Pathways: NF-kappaB Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Stem Cell Maintenance, Hepatitis C, Positive Regulation of Response to DNA Damage Stimulus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation, S100 Proteins