CD20 antibody

Catalog No. ABIN6297751

This Mouse Monoclonal antibody specifically detects CD20 in FACS, IF and Func. It exhibits reactivity toward Human.

Quick Overview for CD20 antibody (ABIN6297751)

Target: CD20 (MS4A1) (Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD20 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Functional Studies (Func)

Product Details anti-CD20 Antibody

Purpose: Mouse anti-Human CD20 Antibody [Sodium Azide Free]

Specificity: Predominantly cell surface with some cytoplasmic

Purification: The lymphoblastoid cell line Daudi was used as the immunogen for this CD20 antibody.

Immunogen: The lymphoblastoid cell line Daudi was used as the immunogen for this CD20 antibody.

Application Details

Application Notes: Functional Testing: order azide-free format
FACS: 0.5-1 μg/million cells
IF: 0.5-1 μg/mL

Restrictions: For Research Use only

Handling

Buffer: In 1X PBS ( pH 7.4), BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Target Details for CD20

Target: CD20 (MS4A1) (Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1))

Alternative Name: B-lymphocyte antigen CD20

Background:

Target Description: This antibody recognizes a protein of 33-37 kDa, identified as CD20 (Workshop V, Code CD20.12). The antibody recognizes the extracellular domain of CD20. The epitope is similar to or identical to that recognized by other CD20 antibodies including Leu-16 and B1. This antibody can be used for immunophenotyping of leukemia and malignant cells, B lymphocyte detection in peripheral blood, Bcell localization in tissues and B lymphocyte purification by immunosorbent methods. CD20 is a non-Ig differentiation antigen of Bcells and its expression is restricted to normal and neoplastic Bcells, being absent from all other leukocytes and tissues. It is expressed by pre Bcells and persists during all stages of Bcell maturation but is lost upon terminal differentiation into plasma cells. Protein passes through the membrane 4 times with both ends in cytoplasm and exposes one short and one longer loop to the external environment. CD20 is not glycosylated in resting Bcells and its cytoplasmic domains are differentially phosphorylated upon activation. It acts as a calcium channel involved in Bcell activation and cell cycle progression.

Gene Symbol: MS4A1///CD20

Gene ID: 931

UniProt: P11836