MCM2 antibody (N-Term)

Catalog No. ABIN630186

The Rabbit Polyclonal anti-MCM2 antibody (ABIN630186) specifically detects MCM2 in WB. The antibody is reactive with Human samples.

Quick Overview for MCM2 antibody (N-Term) (ABIN630186)

Target: MCM2 (Minichromosome Maintenance Complex Component 2 (MCM2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MCM2 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-MCM2 Antibody

Binding Specificity: N-Term

Specificity: MCM2 antibody was raised against the N terminal of MCM2

Purification: Purified

Immunogen: MCM2 antibody was raised using the N terminal of MCM2 corresponding to a region with amino acids PGRSSRRTDALTSSPGRDLPPFEDESEGLLGTEGPLEEEEDGEELIGDGM

Application Details

Application Notes: WB: 1.25 µg/mL
Optimal conditions should be determined by the investigator.

Comment:

MCM2 Blocking Peptide, (ABIN5614697), is also available for use as a blocking control in assays to test for specificity of this MCM2 antibody

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Lyophilized powder. Add distilled water for a 1 mg/mL concentration of MCM2 antibody in PBS

Concentration: Lot specific

Buffer: PBS

Handling Advice: Avoid repeated freeze/thaw cycles.
Dilute only prior to immediate use.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2-8 °C for short periods. For longer periods of storage, store at -20 °C.

Target Details for MCM2

Target: MCM2 (Minichromosome Maintenance Complex Component 2 (MCM2))

Alternative Name: MCM2

Background: The protein encoded by MCM2 is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein forms a complex with MCM4, 6, and 7, and has been shown to regulate the helicase activity of the complex. This protein is phosphorylated, and thus regulated by, protein kinases CDC2 and CDC7.

Molecular Weight: 99 kDa (MW of target protein)

Pathways: DNA Damage Repair, Mitotic G1-G1/S Phases, DNA Replication, Chromatin Binding, Synthesis of DNA