BAG2 antibody (C-Term)

Catalog No. ABIN630239

The Rabbit Polyclonal anti-BAG2 antibody has been validated for WB and IHC. It is suitable to detect BAG2 in samples from Human, Rat, Mouse, Dog and Zebrafish (Danio rerio).

Quick Overview for BAG2 antibody (C-Term) (ABIN630239)

Target: BAG2 (BCL2-Associated Athanogene 2 (BAG2))

Reactivity: Human, Rat, Mouse, Dog, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BAG2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-BAG2 Antibody

Binding Specificity: C-Term

Specificity: BAG2 antibody was raised against the C terminal of BAG2

Purification: Purified

Immunogen: BAG2 antibody was raised using the C terminal of BAG2 corresponding to a region with amino acids VDQKFQSIVIGCALEDQKKIKRRLETLLRNIENSDKAIKLLEHSKGAGSK

Application Details

Application Notes: WB: 1.25 µg/mL, IHC: 4-8 µg/mL
Optimal conditions should be determined by the investigator.

Comment:

BAG2 Blocking Peptide, (ABIN5612271), is also available for use as a blocking control in assays to test for specificity of this BAG2 antibody

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Lyophilized powder. Add distilled water for a 1 mg/mL concentration of BAG2 antibody in PBS

Concentration: Lot specific

Buffer: PBS

Handling Advice: Avoid repeated freeze/thaw cycles.
Dilute only prior to immediate use.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2-8 °C for short periods. For longer periods of storage, store at -20 °C.

Target Details for BAG2

Target: BAG2 (BCL2-Associated Athanogene 2 (BAG2))

Alternative Name: BAG2

Background: BAG proteins compete with Hip for binding to the Hsc70/Hsp70 ATPase domain and promote substrate release. All the BAG proteins have an approximately 45-amino acid BAG domain near the C terminus but differ markedly in their N-terminal regions. The predicted BAG2 protein contains 211 amino acids. The BAG domains of BAG1, BAG2, and BAG3 interact specifically with the Hsc70 ATPase domain in vitro and in mammalian cells. All 3 proteins bind with high affinity to the ATPase domain of Hsc70 and inhibit its chaperone activity in a Hip-repressible manner.

Molecular Weight: 23 kDa (MW of target protein)