Prohibitin antibody (C-Term)

Catalog No. ABIN634116

Product Details anti-Prohibitin Antibody

Target: Prohibitin (PHB)

Binding Specificity: C-Term

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Prohibitin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Specificity: Prohibitin antibody was raised against the C terminal of PHB

Purification: Affinity purified

Immunogen: Prohibitin antibody was raised using the C terminal of PHB corresponding to a region with amino acids AEQQKKAAIISAEGDSKAAELIANSLATAGDGLIELRKLEAAEDIAYQLS

Application Details

Application Notes: WB: 1-2 µg/mL, IHC: 4-8 µg/mL
Optimal conditions should be determined by the investigator.

Comment:

Prohibitin Blocking Peptide, catalog no. 33R-1146, is also available for use as a blocking control in assays to test for specificity of this Prohibitin antibody

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Lyophilized powder. Add distilled water for a 1 mg/mL concentration of PHB antibody in PBS

Concentration: Lot specific

Buffer: PBS

Handling Advice: Avoid repeated freeze/thaw cycles.
Dilute only prior to immediate use.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2-8 °C for short periods. For longer periods of storage, store at -20 °C.

Target Details for Prohibitin

Target: Prohibitin (PHB)

Alternative Name: Prohibitin (PHB Products)

Synonyms: phb1 antibody, MGC75944 antibody, PHB antibody, Phb antibody, ACYPI000080 antibody, phb antibody, PHB1 antibody, Bap32 antibody, sr:nyz128 antibody, wu:fa18f07 antibody, wu:fb09f05 antibody, prohibitin1 antibody, xphb1 antibody, prohibitin antibody, prohibitin-2 antibody, prohibitin L homeolog antibody, PHB antibody, phb antibody, LOC484431 antibody, LOC5576054 antibody, Phb antibody, phb.L antibody

Background: Prohibitin is from an evolutionarily conserved gene that is ubiquitously expressed. It is thought to be a negative regulator of cell proliferation and may be a tumor suppressor. Mutations in PHB have been linked to sporadic breast cancer. Prohibitin is expressed as two transcripts with varying lengths of 3' untranslated region. The longer transcript is present at higher levels in proliferating tissues and cells, suggesting that this longer 3' untranslated region may function as a trans-acting regulatory RNA.

Molecular Weight: 30 kDa (MW of target protein)

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling