Biotin antibody (HRP)

Catalog No. ABIN636126

The HRP-conjugated Goat Polyclonal anti-Biotin antibody (ABIN636126) specifically detects Biotin in WB, IHC and ELISA.

Quick Overview for Biotin antibody (HRP) (ABIN636126)

Target: Biotin

Host: Goat

Clonality: Polyclonal

Conjugate: This Biotin antibody is conjugated to HRP

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Product Details anti-Biotin Antibody (HRP)

Purification: Affinity purified

Purity: > 95 % pure

Immunogen: Biotin
Goat serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.

Application Details

Application Notes: 1:200-1:5000 ELISA/WB, 1:500-1:5000 IHC
Optimal conditions should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: Lot specific

Buffer: Lyophilized powder containing 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, 1 % (w/v) BSA and 0.1 % (v/v) Kathon CG. Protease/IgG free, pH 7.2. Rehydrate with 1.1 mL of deionized water. Prepare fresh working dilution daily.

Preservative: Kathon CG

Precaution of Use: This product contains Kathon CG: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.
Dilute only prior to immediate use.

Storage: 4 °C

Storage Comment: Store lyophilized material at 2-8 °C.

Target Details for Biotin

Target: Biotin

Target Type: Chemical

Background: Biotin is a water-soluble B-complex vitamin (vitamin B7). Biotin is necessary for cell growth, the production of fatty acids, and the metabolism of fats and amino acids. It plays a role in the citric acid cycle, which is the process by which biochemical energy is generated during aerobic respiration. Biotin not only assists in various metabolic reactions but also helps to transfer carbon dioxide. Biotin may also be helpful in maintaining a steady blood sugar level.