AMACR antibody (AA 33-48)

Catalog No. ABIN636371

The Rabbit Polyclonal anti-AMACR antibody has been validated for WB and IHC. It is suitable to detect AMACR in samples from Human.

Quick Overview for AMACR antibody (AA 33-48) (ABIN636371)

Target: AMACR (alpha-Methylacyl-CoA Racemase (AMACR))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This AMACR antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-AMACR Antibody

Binding Specificity: AA 33-48

Purification: Affinity chromatography purified

Immunogen: AMACR antibody was raised in rabbit using AA 33-48 [RVDRPGSRYDVSRLGR] of the 42 kDa human AMACR (P504 S) protein as the immunogen.

Isotype: IgG

Application Details

Application Notes: IHC: 1:100, WB: 1:500. For IHC, an incubation period of 30 minutes at room temperature is suggested. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining.
Optimal conditions should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: Lot specific

Buffer: Affinity purified and supplied in PBS, with 0.02 % NaN3.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles.
Dilute only prior to immediate use.

Storage: 4 °C/-20 °C

Storage Comment: Store at 4 °C for short term storage. Aliquot and store at -20 °C for long term storage.

Target Details for AMACR

Target: AMACR (alpha-Methylacyl-CoA Racemase (AMACR))

Alternative Name: AMACR

Background: Alpha-methylacyl-CoA racemase (AMACR), (also known as P504S) is an enzyme that is involved in peroxisomal beta-oxidation of dietary branched-chain fatty acids. Recent studies have shown that, compared with expression in normal or benign prostate epithelium, AMACR is consistently overexpressed in prostate cancer epithelium, making it a specific marker for cancer cells within the prostate gland.

Pathways: Monocarboxylic Acid Catabolic Process