Biotin antibody

Catalog No. ABIN636380

The Goat Polyclonal anti-Biotin antibody is suitable to detect Biotin. It has been validated for WB, IHC, ELISA and ICC.

Quick Overview for Biotin antibody (ABIN636380)

Target: Biotin

Host: Goat

Clonality: Polyclonal

Conjugate: This Biotin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunocytochemistry (ICC)

Product Details anti-Biotin Antibody

Purification: Affinity chromatography purified

Isotype: IgG

Application Details

Application Notes: ELISA: coating:1:100-1:500, primary:1:1,000-1:30,000, ICC: 1:200-1:2,000,IHC: 1:200-1:2,000, WB: colorimetric: 1:1,000-1:10,000, chemiluminescent: 1:1,000-1:30,000
Optimal conditions should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: Lot specific

Buffer: Affinity purified and supplied in PBS, pH 7.2, with 0.1 % NaN3.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C

Target Details for Biotin

Target: Biotin

Target Type: Chemical

Background: Biotin is a water-soluble B-complex vitamin (vitamin B7) that is composed of a ureido (tetrahydroimidizalone) ring fused with a tetrahydrothiophene ring. A valeric acid substituent is attached to one of the carbon atoms of the tetrahydrothiophene ring. Biotin is a coenzyme in the metabolism of fatty acids and leucine, and it plays a role in gluconeogenesis.