PPP1R12A antibody (C-Term)

Catalog No. ABIN6391349

The Goat Polyclonal anti-PPP1R12A antibody has been validated for IHC, ELISA, IF and FACS. It is suitable to detect PPP1R12A in samples from Human.

Quick Overview for PPP1R12A antibody (C-Term) (ABIN6391349)

Target: PPP1R12A (Myosin Phosphatase, Target Subunit 1 (PPP1R12A))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This PPP1R12A antibody is un-conjugated

Application: Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Grade: Verified

Product Details anti-PPP1R12A Antibody

Binding Specificity: C-Term

Purpose: PPP1R12A

Sequence: REDEYKQKYS RTYD

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Testis. Recommended concentration: 4-5 μg/mL. Preliminary testing showed positive staining of the neuronal cells and neuropils in paraffin embedded Human Cortex.

Peptide ELISA: antibody detection limit dilution 1:8000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the cytoplasm of A431 and U2OS cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration:

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for PPP1R12A

Target: PPP1R12A (Myosin Phosphatase, Target Subunit 1 (PPP1R12A))

Alternative Name: PPP1R12A

Background: Protein phosphatase 1, regulatory (inhibitor) subunit 12A, MBS, MGC133042, MYPT1, myosin phosphatase, target subunit 1

NCBI Accession: NP_002471, NP_001231919, NP_001231921, NP_001137358

Pathways: M Phase