APP antibody (AA 1-16)

Catalog No. ABIN6391362

This anti-APP antibody is a Goat Polyclonal antibody detecting APP in WB and ELISA. Suitable for Human.

Quick Overview for APP antibody (AA 1-16) (ABIN6391362)

Target: APP (Amyloid beta (A4) Precursor Protein (APP))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This APP antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-APP Antibody

Binding Specificity: AA 1-16

Purpose: Amyloid beta peptide (aa1-16)

Sequence: DAEFRHDSGY EVHHQK

Specificity: This antibody is expected to recognize all reported isoforms of APP (NP_000475.1, NP_958816.1, NP_958817.1, NP_001129601.1, NP_001129602.1) and to recognize all lengths of the Amyloid beta peptide.

Cross-Reactivity: Cow, Dog, Human, Pig

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 75 kDa band observed in Human Brain (Cerebral and Frontal cortex) lysates (calculated MW of 78.7 kDa according to NP_958817.1 and 72.6 according to NP_001129601.1). Recommended concentration: 1-3 μg/mL. Primary incubation was 1 hour.

Peptide ELISA: antibody detection limit dilution 1:8000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for APP

Target: APP (Amyloid beta (A4) Precursor Protein (APP))

Alternative Name: APP

Background: APP, amyloid beta (A4) precursor protein, AAA, ABETA, ABPP, AD1, APPI, CTFgamma, CVAP, PN2, OTTHUMP00000096096, PN-II, alzheimer disease amyloid protein, amyloid beta A4 protein, beta-amyloid peptide, cerebral vascular amyloid peptide, peptidase nexin-II,

Gene ID: 351

NCBI Accession: NP_000475, NP_958816, NP_958817, NP_001129601, NP_001129602

Pathways: Caspase Cascade in Apoptosis, EGFR Signaling Pathway, Transition Metal Ion Homeostasis, Skeletal Muscle Fiber Development, Toll-Like Receptors Cascades, Feeding Behaviour