HNF4A antibody (Internal Region)
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- Target See all HNF4A Antibodies
- HNF4A (Hepatocyte Nuclear Factor 4, alpha (HNF4A))
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Binding Specificity
- Internal Region
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Reactivity
- Mouse
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Host
- Goat
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Clonality
- Polyclonal
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Conjugate
- This HNF4A antibody is un-conjugated
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Application
- ELISA, Immunofluorescence (IF)
- Purpose
- HNF4A
- Sequence
- HPHLMQEHMG TN
- Specificity
- This antibody is expected to recognise isoforms 1, 2, 4, 5, 7, 8 and 9
- Cross-Reactivity
- Dog, Human, Mouse, Rat
- Purification
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- Grade
- Verified
- Isotype
- IgG
- Top Product
- Discover our top product HNF4A Primary Antibody
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- Application Notes
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Western Blot: Preliminary experiments showed an approx 55 kDa band in Human Colon, Duodenum, Kidney and Liver lysates (calculated MW of 52.8 kDa according to NP_000448.3). Recommended concentration:0.3-1 μg/mL. Primary incubation 1 hour at room temperature.
Peptide ELISA: antibody detection limit dilution 1:16000.
- Comment
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Immunofluorescence: Expression of the protein seen in the nucleus of NIH3T3 cells. Recommended concentration: 10μg/ml.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.5 mg/mL
- Buffer
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Minimize freezing and thawing.
- Storage
- -20 °C
- Storage Comment
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- Target
- HNF4A (Hepatocyte Nuclear Factor 4, alpha (HNF4A))
- Alternative Name
- HNF4A (HNF4A Products)
- Synonyms
- HNF4A antibody, HNF4alpha antibody, CG9310 antibody, DmHNF4 antibody, Dmel\\CG9310 antibody, HNF-4 antibody, HNF-4(D) antibody, HNF4 antibody, Hnf-4 antibody, Hnf-4h antibody, NR2A4 antibody, dHNF-4 antibody, dHNF4 antibody, Hnf4 antibody, Hnf4alpha antibody, MODY1 antibody, Nr2a1 antibody, Tcf14 antibody, HNF4a7 antibody, HNF4a8 antibody, HNF4a9 antibody, MODY antibody, NR2A1 antibody, NR2A21 antibody, TCF antibody, TCF14 antibody, HNF-4alpha antibody, fb58h09 antibody, id:ibd1279 antibody, wu:fb58h09 antibody, hnf4 antibody, nr2a1 antibody, hepatocyte nuclear factor 4 alpha antibody, Hepatocyte nuclear factor 4 antibody, hepatic nuclear factor 4, alpha antibody, hepatocyte nuclear factor 4, alpha antibody, hepatocyte nuclear factor 4 alpha S homeolog antibody, HNF4A antibody, hnf4a antibody, Hnf4 antibody, Hnf4a antibody, hnf4a.S antibody
- Background
- HNF4A, hepatocyte nuclear factor 4, alpha , FLJ39654, HNF4, HNF4a7, HNF4a8, HNF4a9, MODY, MODY1, NR2A1, NR2A21, TCF, TCF14, HNF4-alpha, hepatic nuclear factor 4 alpha, hepatocyte nuclear factor 4 alpha, transcription factor-14
- Gene ID
- 3172
- NCBI Accession
- NP_849180, NP_000448, NP_787110, NP_001025174, NP_001245284, NP_001274111, NP_001274112
- Pathways
- AMPK Signaling, Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Carbohydrate Homeostasis, Cell-Cell Junction Organization, Regulation of Carbohydrate Metabolic Process
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