HNF4A antibody (Internal Region)

Catalog No. ABIN6391399

This Goat Polyclonal antibody specifically detects HNF4A in ELISA and IF. It exhibits reactivity toward Mouse.

Quick Overview for HNF4A antibody (Internal Region) (ABIN6391399)

Target: HNF4A (Hepatocyte Nuclear Factor 4, alpha (HNF4A))

Reactivity: Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This HNF4A antibody is un-conjugated

Application: ELISA, Immunofluorescence (IF)

Grade: Verified

Product Details anti-HNF4A Antibody

Binding Specificity: Internal Region

Purpose: HNF4A

Sequence: HPHLMQEHMG TN

Specificity: This antibody is expected to recognise isoforms 1, 2, 4, 5, 7, 8 and 9

Cross-Reactivity: Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Preliminary experiments showed an approx 55 kDa band in Human Colon, Duodenum, Kidney and Liver lysates (calculated MW of 52.8 kDa according to NP_000448.3). Recommended concentration:0.3-1 μg/mL. Primary incubation 1 hour at room temperature.

Peptide ELISA: antibody detection limit dilution 1:16000.

Comment:

Immunofluorescence: Expression of the protein seen in the nucleus of NIH3T3 cells. Recommended concentration: 10μg/ml.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for HNF4A

Target: HNF4A (Hepatocyte Nuclear Factor 4, alpha (HNF4A))

Alternative Name: HNF4A

Background: HNF4A, hepatocyte nuclear factor 4, alpha , FLJ39654, HNF4, HNF4a7, HNF4a8, HNF4a9, MODY, MODY1, NR2A1, NR2A21, TCF, TCF14, HNF4-alpha, hepatic nuclear factor 4 alpha, hepatocyte nuclear factor 4 alpha, transcription factor-14

Gene ID: 3172

NCBI Accession: NP_849180, NP_000448, NP_787110, NP_001025174, NP_001245284, NP_001274111, NP_001274112

Pathways: AMPK Signaling, Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Carbohydrate Homeostasis, Cell-Cell Junction Organization, Regulation of Carbohydrate Metabolic Process