TNF alpha antibody (Biotin)

Catalog No. ABIN639287

The Mouse Monoclonal anti-TNF alpha antibody has been validated for ELISA and FACS. It is suitable to detect TNF alpha in samples from Cow.

Quick Overview for TNF alpha antibody (Biotin) (ABIN639287)

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Reactivity: Cow

Host: Mouse

Clonality: Monoclonal

Conjugate: This TNF alpha antibody is conjugated to Biotin

Application: ELISA, Flow Cytometry (FACS)

Product Details anti-TNF alpha Antibody (Biotin)

Purpose: Mouse Anti-Bovine TNF-alpha [+Biotin]

Specificity: This product recognizes bovine TNF alpha, a 17.5kDa cytokine, expressed by many different stimulated cell types including monocytes, macrophages, endothelial cells, fibroblasts and both T and B-lymphocytes. The production of TNF alpha is induced by a variety of factors, dependant upon cell type and includes bacterial toxins, IL-1, PDGF, IFN-beta, NGF, Oncostatin M and viral infections. The presence of TNF alpha is responsible for diverse immunomodulatory, anti-tumour and toxic effects and under certain conditions is also capable of self-stimulation and inhibition. This biotin conjugate may be used as a detection antibody in ELISA assays for bovine TNF alpha in combination with DS-MB-02436 as capture reagent.

Cross-Reactivity: Sheep (Ovine)

Characteristics: Cytokines/Chemokines/Growth Factors/Receptors

Immunogen: Recombinant bovine TNF alpha

Isotype: IgG2a

Application Details

Application Notes: ELISA (dilution: 1/10 , 1/100), Flow Cytometry

Restrictions: For Research Use only

Handling

Concentration: IgG concentration 1.0 mg/ml

Buffer: Phosphate buffered saline pH7.4

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: -20°C

Target Details for TNF alpha

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Alternative Name: Tumor Necrosis Factor alpha (TNF-alpha)

Pathways: NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, TLR Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome