alpha Tubulin antibody

Catalog No. ABIN640556

The Rat anti-alpha Tubulin antibody has been validated for WB, ELISA, IP, IHC (fro) and RIA. It is suitable to detect alpha Tubulin in samples from Saccharomyces cerevisiae.

Quick Overview for alpha Tubulin antibody (ABIN640556)

Target: alpha Tubulin (TUBA1)

Reactivity: Saccharomyces cerevisiae

Host: Rat

Conjugate: This alpha Tubulin antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Radioimmunoassay (RIA)

Product Details anti-alpha Tubulin Antibody

Specificity: DS-MB-02478 recognizes the alpha subunit of tubulin, specifically binding tyrosylated Tubulin (Tyr-Tubulin) (2). The epitope recognised by this antibody has been extensively studied and would appear to be a linear sequence requiring an aromatic residue at the C terminus, with the two adjacent amino acids being negatively charged (represented by Gly-Gly-Tyr in Tyr-Tubulin). The antibody has been used in epitope tagging procedures to detect proteins tagged with a C-terminal Gly-Gly- Phe epitope. These sequence requirements have been reported to result in some cross-reactivity with other proteins in certain circumstances, including E. coli rec A and oxidized actin (5). This product is routinely tested in ELISA on Tubulin. Reacts with: Yeast, Mammals, Birds. Antibody reactivity and working conditions may vary between species.

Characteristics: Rat Anti-Tubulin alpha

Purification: Purified

Immunogen: Yeast tubulin

Isotype: IgG2a

Application Details

Restrictions: For Research Use only

Handling

Concentration: IgG concentration 1.0 mg/ml

Buffer: Phosphate buffered saline pH7.4

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Target Details for alpha Tubulin

Target: alpha Tubulin (TUBA1)

Alternative Name: Tubulin alpha

Pathways: Microtubule Dynamics