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MED25 antibody (AA 112-140)

This anti-MED25 antibody is a Rabbit Polyclonal antibody detecting MED25 in WB and IHC (p). Suitable for Human and Mouse. This Primary Antibody has been cited in 1 publication.
Catalog No. ABIN654162

Quick Overview for MED25 antibody (AA 112-140) (ABIN654162)

Target

See all MED25 Antibodies
MED25 (Mediator Complex Subunit 25 (MED25))

Reactivity

  • 21
  • 13
  • 5
  • 4
  • 4
  • 4
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
Human, Mouse

Host

  • 21
Rabbit

Clonality

  • 21
Polyclonal

Conjugate

  • 12
  • 3
  • 2
  • 2
  • 1
  • 1
This MED25 antibody is un-conjugated

Application

  • 15
  • 11
  • 8
  • 2
  • 2
  • 2
  • 1
Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone

RB24225
  • Binding Specificity

    • 12
    • 4
    • 2
    • 2
    • 1
    • 1
    AA 112-140

    Predicted Reactivity

    B

    Purification

    This antibody is purified through a protein A column, followed by peptide affinity purification.

    Immunogen

    This MED25 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 112-140 amino acids of human MED25.

    Isotype

    IgG
  • Application Notes

    WB: 1:1000. WB: 1:1000. IHC-P: 1:50~100

    Restrictions

    For Research Use only
  • Format

    Liquid

    Buffer

    Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

    Preservative

    Sodium azide

    Precaution of Use

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Storage

    4 °C,-20 °C

    Storage Comment

    Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

    Expiry Date

    6 months
  • Han, Rha, Chi: "MED25 is a mediator component of HNF4α-driven transcription leading to insulin secretion in pancreatic beta-cells." in: PLoS ONE, Vol. 7, Issue 8, pp. e44007, (2013) (PubMed).

  • Target

    MED25 (Mediator Complex Subunit 25 (MED25))

    Alternative Name

    MED25

    Background

    This gene encodes a component of the transcriptional coactivator complex termed the Mediator complex. This complex is required for transcription of most RNA polymerase II-dependent genes. The encoded protein plays a role in chromatin modification and in preinitiation complex assembly. Mutations in this gene are associated with Charcot-Marie-Tooth disease type 2B2. [provided by RefSeq].

    Molecular Weight

    78171

    Gene ID

    81857

    NCBI Accession

    NP_112235

    UniProt

    Q71SY5

    Pathways

    Nuclear Hormone Receptor Binding, Chromatin Binding, Regulation of Lipid Metabolism by PPARalpha, Production of Molecular Mediator of Immune Response
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