LEF1 antibody (N-Term)

Catalog No. ABIN655987

Product Details anti-LEF1 Antibody

Target: LEF1 (Lymphoid Enhancer-Binding Factor 1 (LEF1))

Binding Specificity: AA 10-37, N-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LEF1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Predicted Reactivity: M, Rat

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This LEF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 10-37 amino acids from the N-terminal region of human LEF1.

Clone: RB31989

Isotype: Ig Fraction

Application Details

Application Notes: IF: 1:10~50. WB: 1:2000. WB: 1:2000. WB: 1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Expiry Date: 6 months

References for anti-LEF1 antibody (ABIN655987)

Yao, Li, Liang, Lu, Zhu, Liu, Zhang, Yuan, Wang: "Methylprednisolone suppresses the Wnt signaling pathway in chronic lymphocytic leukemia cell line MEC-1 regulated by LEF-1 expression." in: International journal of clinical and experimental pathology, Vol. 8, Issue 7, pp. 7921-8, (2015) (PubMed).

Target Details for LEF1

Target: LEF1 (Lymphoid Enhancer-Binding Factor 1 (LEF1))

Alternative Name: LEF1 (LEF1 Products)

Background: This gene encodes a transcription factor belonging to a family of proteins that share homology with the high mobility group protein-1. The protein encoded by this gene can bind to a functionally important site in the T-cell receptor-alpha enhancer, thereby conferring maximal enhancer activity. This transcription factor is involved in the Wnt signaling pathway, and it may function in hair cell differentiation and follicle morphogenesis. Mutations in this gene have been found in somatic sebaceous tumors. This gene has also been linked to other cancers, including androgen-independent prostate cancer. Alternative splicing results in multiple transcript variants.

Molecular Weight: 44201

Gene ID: 51176

NCBI Accession: NP_001124185, NP_001124186, NP_001159591, NP_057353

UniProt: Q9UJU2

Pathways: WNT Signaling, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Hormone Metabolic Process, Nuclear Hormone Receptor Binding, Chromatin Binding