Braf V600E antibody

Details for Product No. ABIN6560108
Antigen
Reactivity
Human
3
1
Host
Rabbit
4
Antibody Type
Recombinant Antibody
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
1
Application
Immunocytochemistry (ICC), Immunohistochemistry (IHC), Western Blotting (WB)
3
1
1
1
1
Options
Immunogen A peptide corresponding to BRAF V600E mutant was used as the immunogen for the recombinant BRAF V600E antibody.
Clone RM8
Isotype IgG
Characteristics This antibody reacts to the BRAF V600E mutant. No cross reactivity seen with wild type BRAF.
Purification Purified
Purity Protein A purified from animal origin-free supernatant
Antigen
Background This antibody reacts to the BRAF V600E mutant. No cross reactivity seen with wild type BRAF.
UniProt P15056
Application Notes The stated application concentrations are suggested starting points. Titration of the recombinant BRAF V600E antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Immunohistochemistry: (FFPE): 0.5-5 μg/mL,Immunocytochemistry: 2-10 μg/mL,Western Blot: 0.5-2 μg/mL
Restrictions For Research Use only
Buffer 1 mg/mL in PBS with 50 % Glycerol, 1 % BSA and 0.09 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store the recombinant BRAF V600E antibody at -20°C.
Supplier Images
Image no. 1 for anti-Braf V600E antibody (ABIN6560108) Western blot analysis of cell lysates prepared from cell lines expressing endogenous ...
Image no. 2 for anti-Braf V600E antibody (ABIN6560108) IHC staining of formalin fixed and paraffin embedded thyroid carcinoma tissue with re...
Image no. 3 for anti-Braf V600E antibody (ABIN6560108) ICC staining of formalin fixed and paraffin embedded human WiDr cells (400X, Positive...
Image no. 4 for anti-Braf V600E antibody (ABIN6560108) IHC staining of formalin fixed and paraffin embedded thyroid carcinoma tissue with re...
Image no. 5 for anti-Braf V600E antibody (ABIN6560108) ICC staining of formalin fixed and paraffin embedded human SW40 cells (400X, Negative...